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Rapid Detection of Ganoderma Disease of Coconut by using ITS-PCR and Assessment of Inhibition Effect of Various Control Measures by Fungicides and Bioagents

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Title Rapid Detection of Ganoderma Disease of Coconut by using ITS-PCR and Assessment of Inhibition Effect of Various Control Measures by Fungicides and Bioagents
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Creator J. Raju, S. T. Naik, S. Priti, V. Suryanarayana, V. I. Benagi, Jones Nirmalanath and Madhu S. Giri
 
Subject Biochemical, Molecular, diagnosis, Ganoderma, Bioagents, fungicides
 
Description Not Available
Two biochemical and molecular methods have been applied for detecting the
Ganoderma, basidiomycete fungi causing basal stem rot disease (BSR) of coconut in the
early stage itself. Leaf, stem/bark and root extracts recorded the highest optical density values
than their corresponding healthy tissues. Increased transmittance values observed from
the extracts of diseased leaf, stem, bark and root extracts compared healthy tissues. For
polymerase chain reaction (PCR) test, the primer generated from the internal transcribed
spacer region one (ITS 1) of ribosomal DNA gene of Ganoderma, which produced a PCR
product of 160 bp in size is used for early detection. Different systemic, non-systemic and
bioagents were screened against Ganoderma isolates for in vitro management of the
disease. Among the non-systemic fungicides tried in vitro, captan, mancozeb, and thiram
at 0.2 percent completely inhibited the growth of the fungus. In the case of systemic fungicides
trideomarphm, hexaconzole and carbendazim at 0.1 percent recorded highest percent
inhibition of mycelium growth. Dual culture study carried out with three different species
of Trichoderma revealed that T. harzianum and T. koningii were found effective.
Not Available
 
Date 2019-10-15T10:05:21Z
2019-10-15T10:05:21Z
2015-09-01
 
Type Research Paper
 
Identifier Not Available
0973-7510
http://krishi.icar.gov.in/jspui/handle/123456789/23409
 
Language English
 
Relation Not Available;
 
Publisher Not Available