Record Details

Structure of triosephosphate isomerase (TIM) from Methanocaldococcus jannaschii

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Structure of triosephosphate isomerase (TIM) from Methanocaldococcus jannaschii
Not Available
 
Creator Gayathri, P., Banerjee, M., Vijayalakshmi, A., Azeez, S., Balaram, H., Balaram, P. and Murthy, M.R.N.
 
Subject triosephosphate isomerase (TIM); Methanocaldococcus jannaschii; tetrameric TIM; tetartohedral twinning; disordered loop 6
 
Description Not Available
The crystal structure of a recombinant triosephosphate isomerase (TIM) from the archaeabacterium Methanocaldococcus jannaschii has been determined at a resolution of 2.3 A using X-ray diffraction data from a tetartohedrally twinned crystal. M. jannaschii TIM (MjTIM) is tetrameric, as suggested by solution studies and from the crystal structure, as is the case for two other structurally characterized archaeal TIMs. The archaeabacterial TIMs are shorter compared with the dimeric TIMs; the insertions in the dimeric TIMs occur in the vicinity of the tetramer interface, resulting in a hindrance to tetramerization in the dimeric TIMs. The charge distribution on the surface of the archaeal TIMs also facilitates tetramerization. Analysis of the barrel interactions in TIMs suggests that these interactions are unlikely to account for the thermal stability of the archaeal TIMs. A novelty of the unliganded structure of MjTIM is the complete absence of electron density for the loop 6 residues. The disorder of this loop could be ascribed to a missing salt bridge between residues at the N- and C-terminal ends of the loop in MjTIM.
Not Available
 
Date 2019-10-16T10:07:25Z
2019-10-16T10:07:25Z
2007-01-29
 
Type Research Paper
 
Identifier Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/23489
 
Language English
 
Relation Not Available;
 
Publisher Europe PMC