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Enhancement of RNAi by using concatemerized double-stranded RNA

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Title Enhancement of RNAi by using concatemerized double-stranded RNA
Not Available
 
Creator Sharath Chandra, G; Asokan, R; Manamohan, M.
 
Subject RNA interference; dsRNA concatemers; Plutella xylostella; acetylcholinesterase; RNAi pathway genes
 
Description Not Available
BACKGROUND: RNA interference (RNAi) is a potential tool for functional characterization of genes and also in the management
of insect pests. Accumulated literature reveals that the RNAi efficiency varies among insect species and is reported to be less
efficient in lepidopteran insects.
RESULTS: We attempted to enhance RNAi efficiency by concatemerizing short double-stranded RNA (dsRNA) sequence. Then
the effectiveness of concatemerized dsRNAs (C-dsRNAs) was compared with non-concatemerized long dsRNA (NCL-dsRNA) in
silencing of acetylcholinesterase (AChE) in the diamondback moth, Plutella xylostella (Lepidoptera), amajor pest on cruciferous
vegetables. Results revealed that the C-dsRNAs enhanced the RNAi efficiency in terms of higher target gene silencing and
consequently resulted in lower larval weight gain and highermortality compared to the NCL-dsRNA treatment. Even the lower
concentration (0.5 𝛍g) of C-dsRNAs had a relatively similar effect to that of higher concentrations (2 𝛍g) of NCL-dsRNA. The
enhanced RNAi efficiency with C-dsRNAs was plausibly due to the higher expression of core RNAi pathway genes, Dicer-2 and
Argonaute-2 (Ago-2), which are known to govern the efficiency of RNAi.
CONCLUSION:Overall, C-dsRNA enhanced the RNAi activity over routinely used long dsRNA in P. xylostella and this strategymay
be applied for effectivemanagement of insect pests.
Not Available
 
Date 2019-10-18T08:05:07Z
2019-10-18T08:05:07Z
2018-05-01
 
Type Article
 
Identifier Not Available
1526-4998
http://krishi.icar.gov.in/jspui/handle/123456789/23575
 
Language English
 
Relation Not Available;
 
Publisher Wiley