Evaluation of molecular diversity of ex situ conserved germplasm of palmyrah (Borassus flabellifer L.) accessions using RAPD markers
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Title |
Evaluation of molecular diversity of ex situ conserved germplasm of palmyrah (Borassus flabellifer L.) accessions using RAPD markers
Not Available |
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Creator |
Jiji George Anitha Karun
K.T. Venkataramana P. Nainar M.K. Rajesh Anitha Karun |
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Subject |
Borassus flabellifer
genetic diversity germplasm RAPD |
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Description |
Not Available
The genetic relationship of 96 palmyrah palms, consisting of 24 indigenous accessions from Tamil Nadu and Andhra Pradesh, was investigated using Random Amplified Polymorphic DNA (RAPD) markers. Hundred and eighty primers were used initially to identify the polymorphic primers in six random samples and 10 polymorphic primers were selected to amplify the 96 palms. These 10 primers produced a total of 112 reproducible bands and out of them, 41 fragments (36.6 %) showed polymorphism. The number of bands produced with each primer varied from seven to 15 with an average of 11.2 bands per primer. The percent polymorphism ranged from 7.7 to 71.4 with an average of 37.4 per cent when all the primers were taken collectively. UPGMA grouped all the accessions into two major clusters at 0.85 similarity value. The highest similarity value (0.96) was observed between the accessions KLKM-8 and THY-54 and the lowest similarity value (0.782) was obtained between ANBI-17 and RCML-11. The relatively low polymorphism suggests a narrow genetic diversity of palmyrah populations from which the present accessions have been derived and maintained over the years. Not Available |
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Date |
2019-10-18T08:52:48Z
2019-10-18T08:52:48Z 2016-05-01 |
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Type |
Article
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Identifier |
Journal of Plantation Crops, 2016, 44(2): 96-102
2454-8480 http://krishi.icar.gov.in/jspui/handle/123456789/23584 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Indian Society of Plantation Crops
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