Record Details

Duplex PCR to detect both Papaya ring spot virus and Papaya leaf curl virus simultaneously from naturally infected papaya (Carica papaya L.)

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Duplex PCR to detect both Papaya ring spot virus and Papaya leaf curl virus simultaneously from naturally infected papaya (Carica papaya L.)
Not Available
 
Creator Usharani, TR
Laxmi, V
Jalali, S
Krishnareddy, M
 
Subject Papaya leaf curl, papaya ring spot, Duplex PCR
 
Description Not Available
Papaya, a major fruit crop in India and worldwide, is affected by many fungal and viral diseases. A mixed infection of Papaya ring spot virus (PRSV), a linear single-stranded ( + ) RNA genome of approx 10 kb size, and Papaya leaf curl virus (PaLCV), a bipartite Gemini virus (component A & B) having circular single-stranded DNA ( + ) genome of about 5.2 kb, has hampered the production and productivity of papaya in many parts of world. Rapid detection techniques are important in prevention of spread of the disease in field conditions. In the present study, a rapid and reliable PCR based detection protocol has been standardized. Sets of primers were designed, based on the respective virus isolate sequence data available in GenBank, to obtain anticipated products of calculated size.
Not Available
 
Date 2019-10-25T05:19:53Z
2019-10-25T05:19:53Z
2013-04-01
 
Type Research Paper
 
Identifier 11
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/23882
 
Language English
 
Relation Not Available;
 
Publisher NISCAIR-CSIR, India