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Identification of mixed infection caused by Badnavirus and CMV in Jasmine (Jasminum multiflorum Roth)

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Title Identification of mixed infection caused by Badnavirus and CMV in Jasmine (Jasminum multiflorum Roth)
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Creator Usharani, T R Jalali, Salil Manasa, M Samuel, D K Krishnareddy, Mble
 
Subject jasmine, mixed infection, CMV, Badna
 
Description Not Available
The symptoms of chlorotic ring spots and irregular chlorotic patches were recently observed on leaves of landscape-grown jasmine (Jasminum multiflorum Roth) in southern parts of India. The causal agent was mechanically transmitted from symptomatic leaves of jasmine to Nicotiana glutinosa, Capsicum annum, Solanum lycopersicum, Cucurbita pepo and Cucumis sativus. Leaf dip preparations from virus-infected plants in electron microscopy revealed the presence of isometric particles similar to Cucumber mosaic virus (CMV) and bacilliform virus particles of badnavirus. The virus reacted specifically with IgG for CMV and Banana streak virus (BSV) in direct antigen coating, enzyme-linked immunosorbent assay. No reaction was observed with ilar-, poty- and tospo-viruses specific IgG. Reverse transcription polymerase chain reaction with total RNA isolated from symptomatic jasmine leaves and infected N. tabacum 'Xanthi' using CMV coat protein (CMV CP) specific primers amplified the expected product. The association of badna virus with jasmine was confirmed by PCR amplification, cloning and sequencing of 560 bp amplicon corresponding to the reverse transcriptase and ribonuclease H coding region in open reading frame III. The sequence analysis revealed maximum identity to badna virus group Diascorea bacilliform virus (88.5% at nt level) and CMV group IB (96% at nt level). To our knowledge, this is the first report of CMV and badna mixed infection in jasmine in India.
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Date 2019-10-19T09:48:44Z
2019-10-19T09:48:44Z
2016-07-01
 
Type Research Paper
 
Identifier Not Available
0975-0967 (Online); 0972-5849 (Print)
http://krishi.icar.gov.in/jspui/handle/123456789/23662
 
Language English
 
Relation Not Available;
 
Publisher NISCAIR-CSIR, India