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Differentiation of Phytophthora species associated with plantation crops using PCR and high-resolution melting curve analysis

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Title Differentiation of Phytophthora species associated with plantation crops using PCR and high-resolution melting curve analysis
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Creator Prathibha, V.H
Vinayaka Hegde
Sharadraj, K.M
Rajesh, M.K
Rachana, K.E
Chowdappa, P
 
Subject Coconut
Arecanut palm
Phytophthora
HRM analysis
Rapid detection
PCR
 
Description Not Available
Diseases caused by closely related species of Phytophthora such as P. palmivora, P. nicotianae, P. capsici, P. meadii, P. citrophthora and P. colocasiae are major production constraints of plantation crops in India. A reliable method for the rapid identification of these Phytophthora species is required for production of disease-free planting material and promote exports. A specific PCR assay with primer pairs designed from the ITS region effectively differentiated Phytophthora species belonging to different clades viz., P. palmivora, P. nicotianae and P. capsici. In order to differentiate closely related species (P. meadii, P. citrophthora and P. colocasiae), a high-resolution melting (HRM) curve analysis, using primers designed based on single nucleotide differences in A/T and C/A alleles was developed and validated. HRM curve analysis proved to be a fast and accurate technique for differentiation of closely related species of Phytophthora.
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Date 2019-11-01T09:13:32Z
2019-11-01T09:13:32Z
2018-01-01
 
Type Article
 
Identifier Journal of Plant Pathology,100(2):233-240
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http://krishi.icar.gov.in/jspui/handle/123456789/24253
 
Language English
 
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Publisher Not Available