Record Details

In vitro regeneration of coconut plantlets from immature inflorescence

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title In vitro regeneration of coconut plantlets from immature inflorescence
Not Available
 
Creator M. Shareefa, Regi J. Thomas, J. S. Sreelekshmi, M. K. Rajesh and Anitha Karun
 
Subject coconut, in vitro regeneration, immature inflorescence
 
Description Clonal propagation of elite, disease-free coconut
palms is a promising technique for producing uniform
planting material with high yield and disease
resistance. Over the past few decades cloning of coconut
has been attempted in a number of laboratories
worldwide; however, success has been limited. In the
present study, immature inflorescences of 2–12 cm
size were collected from West Coast Tall variety and
the rachilla segments were cultured on four different
media combinations in dark condition. White
translucent outgrowths were maximum in Y3 medium
supplemented with 4.54 μM 2,4-dichlorophenoxyacetic
acid (92%) followed by medium 72 with 41.4 μM
picloram, 61.8 μM putrescine and 4.54 μM thidiazuron
(TDZ) (87%). After eight weeks in dark, shoot-like
outgrowth was noticed more in Y3 III (65%) followed
by Y3 I. After eight months dark incubation, the
cultures were transferred to 1/2 Murashige and Skoog
(MS) with two hormone combinations and high
frequency of multiple shoot formation was noticed in
1/2 MS with 5.37 μM naphthalene acetic acid (NAA)
and 4.44 μM 6-benzylaminopurine (BAP). Maximum
shoot development was observed Y3 medium fortified
with 5 μM 2-isopentenyl adenine (2ip) and 5 μM BAP.
The individual shoots after development of 3–4 leaves
were transferred to 1/2 Y3 medium supplemented
with 5.37 μM NAA and 24.6 μM indole-3-butyric acid
(IBA), and root initiation was observed in 39.28%
plantlets. Start codon targeted (SCoT) profiling based
on banding pattern of PCR-amplified products
confirmed the clonal fidelity of in vitro regenerated
coconut plantlets. The study indicates the possibility
of developing an in vitro regeneration protocol for
coconut using immature inflorescence explants.
Not Available
Not Available
 
Date 2019-11-07T09:39:41Z
2019-11-07T09:39:41Z
2019-09-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/24569
 
Language English
 
Relation Not Available;
 
Publisher Current science