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Organogenesis and anatomical study of gamma rays induced mutant of chrysanthemum (Chrysanthemum morifolium Ramat.) from ray florets

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Title Organogenesis and anatomical study of gamma rays induced mutant of chrysanthemum (Chrysanthemum morifolium Ramat.) from ray florets
Not Available
 
Creator Verma, A.K. and Prasad, K.V.
 
Subject Chrysanthemum, Histological analysis, In vitro, Organogenesis, Ray florets, Regeneration.
 
Description Not Available
We developed an efficient in vitro regeneration
protocol for the isolation, rapid multiplication and field
establishment of Pink Incurving novel mutant from ray
florets of chrysanthemum. Earliest (8.33 ± 1.17 d) and
highest (86.67 ± 2.03%) callogenesis was observed on
Murashige and Skoog (MS) medium supplemented with
10 mg l–1 kinetin (KIN) and 2 mg l–1 α-Naphthalene
acetic acid (NAA). MS medium supplemented with 10
mg l–1 KIN + 0.1 mg l–1 NAA was found most effective
to induce maximum regeneration of micro shoots
(96.33 ± 1.95%) while maximum shoot proliferation
(98.33 ± 0.88%) was obtained when the medium was
supplemented with 5 mg l–1 KIN + 0.01 mg l–1 NAA +
0.2 mg l–1 GA3. Good shoot elongation was noticed
after 30 days of transfer onto a medium comprising of
0.50 mg l–1 GA3. The elongated microshoots were best
rooted on half-strength MS medium supplemented with
0.5 mg l–1 NAA. The rooted plantlets were successful
acclimatized in 3 weeks in glass jar with polypropylene
cap filled with peat + Soilrite® (1:1) and transferred
to greenhouse for flowering.
The plants obtained through this technique produced
the true-to-type flowers in υM2 generation. The isolated
mutant was found stable for the selected characters.
Histological investigations revealed that unorganized
callus got organized and turned into meristematic zone
to produce shoot primordia and further developed into
multiple shoots as the concentration of growth
regulators changed.
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Date 2020-01-10T05:57:36Z
2020-01-10T05:57:36Z
2019-03-01
 
Type Article
 
Identifier Verma, A.K. and Prasad, K.V. (2019). Organogenesis and anatomical study of gamma rays induced mutant of chrysanthemum (Chrysanthemum morifolium Ramat.) from ray florets. Research Journal of Biotechnology, 14(3): 44-53.
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http://krishi.icar.gov.in/jspui/handle/123456789/29460
 
Language English
 
Relation Not Available;
 
Publisher Not Available