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Assessment of diversity amongst natural populations of Gymnema sylvestre from India and development of a validated HPLC protocol for identification and quantification of gymnemagenin

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Title Assessment of diversity amongst natural populations of Gymnema sylvestre from India and development of a validated HPLC protocol for identification and quantification of gymnemagenin
Not Available
 
Creator Tushar Dhanani
Singh R.
Ajit Arun Waman
Patel P
Manivel P
Satyanshu Kumar
 
Subject Genetic diversity
Madhunashini
Antidiabetic
Secondary metabolites
Standard operating protocol
 
Description Not Available
In order to supply uniform, genuine, good quality raw material to the pharmaceutical industries and to reduce the burden on natural stocks, cultivation of medicinal crops is the need of the hour. However, improved varieties with optimum levels of active ingredients have so far not been developed in a number of important medicinal crops. Considering this, an investigation was undertaken to assess the extent of variability amongst the natural populations of Gurmar (Gymnema sylvestre), an important anti-diabetic plant of the Indian Systems of Medicine. Evaluation of the accessions for morphological parameters revealed highly significant differences for leaf related parameters viz. length, width, fresh as well as dry weight and petiole length, which directly contribute to the biological yield of the plant. Further, a validated high performance liquid chromatography-diode array detection (HPLC-DAD) method was also developed for identification and quantification of bioactive principle i.e., gymnemagenin in a gradient elution mode using solvent mixture composing of acetonitrile (solventA), potassium dihydrogenorthophosphate(10mM,solvent B) both solvent A and B containing orthophosphoric acid(0.05%,v/v). Results revealed significant differences for gymnemagenin content amongst the accessions evaluated. These accessions could be used in breeding programs for development of cultivars with optimum levels of gymnemagenin, which in turn may promote the cultivation of this high value medicinal crop.
Not Available
 
Date 2020-01-09T12:30:33Z
2020-01-09T12:30:33Z
2015-12-01
 
Type Research Paper
 
Identifier Dhanani T., Singh R., Ajit Arun Waman, Patel P., Manivel P. and Kumar S., 2015, Assessment of diversity amongst natural populations of Gymnema sylvestre from India and development of a validated HPLC protocol for identification and quantification of gymnemagenin. Industrial Crops and Products, 77: 901-909.
0926-6690
http://krishi.icar.gov.in/jspui/handle/123456789/29439
 
Language English
 
Relation Not Available;
 
Publisher Elsevier B.V.