Record Details


Field	Value

Title	In vitro regeneration of tuberose through petals and immature flower buds Not Available

Creator	G.B. Kadam, K.P. Singh, A.K. Singh, R. Jyothi

Subject	Bulb, flower bud, in vitro regeneration, petal, tuberose.

Description	Not Available Unlike many other bulbous flower crops, where in vitro propagation techniques have been perfected, tuberose does not have enough background studies. A study was carried out to establish aseptic cultures and to standardize in vitro propagation protocol with petal segment and immature flower bud as explants. The explants pre-treated with carbendazim 0.1% + mancozeb 0.1% and 8-HQC (200 mg l-1) for 2½ h and surface sterilized with HgCl2 (0.1%) + NaOCl (1.0%) for 8 min., respectively were found to be the best in getting aseptic culture. The best medium for shoot multiplication was MS medium supplemented with 6.0 mg/l BAP + 0.5 mg/l NAA + 0.7 mg/l 2, 4-D + 0.5 mg/l TDZ for both petals (4.0) and immature flower bud (4.33) enhanced shoot multiplication. Irrespective of explants, maximum roots were observed on half-strength MS medium + 1.0 mg/l IBA. The in vitro regenerated micro-plant lets were successfully acclimatized in glass jar with polypropylene cap. Not Available

Date	2020-03-17T08:05:56Z 2020-03-17T08:05:56Z 2010-03

Type	Article

Identifier	Not Available Not Available http://krishi.icar.gov.in/jspui/handle/123456789/33869

Language	English

Relation	Not Available;

Publisher	Not Available

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