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Role of sterilants in establishment of aseptic culture using different explants in tuberose (Polianthes tuberosa Linn.)

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Title Role of sterilants in establishment of aseptic culture using different explants in tuberose (Polianthes tuberosa Linn.)
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Creator Ganesh. B. Kadam, Krisahn P. Singh, R. Jyothi
 
Subject tuberose, explants
 
Description Not Available
The aim of the present study was to establish an efficient protocol for acquiring aseptic culture from Single and
Double cultivars of tuberose (Polianthes tuberosa Linn.) namely, Prajwal and Vaibhav, respectively. Initially aseptic cultures for bulb scale, buds, petal segment and immature flower bud explants were established by pre-treatment using 0.1 per cent of carbendazim and mancozeb and 200 mg l-1 8-HQC for 4h and 2h, respectively. The survival percent observed was 89.20 per cent and 82.5 per cent in case of bulb scales and bud explants and 87.50 percent and 90.83 percent for flower based explants respectively. HgCl2 and NaOCl applied at 1.0% for 8 min as surface sterilant has shown better results with respect to all the explants used in order to get the good amount of aseptic cultures in tuberose cultivars.
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Date 2020-03-17T08:05:27Z
2020-03-17T08:05:27Z
2010-11-15
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/33867
 
Language English
 
Relation Not Available;
 
Publisher Not Available