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Circumventing phenolic exudation and poor survival in micropropagation of marigold

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Title Circumventing phenolic exudation and poor survival in micropropagation of marigold
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Creator K. Ravindra Kumar, Kanwar Pal Singh, D.V.S. Raju, Prabhat Kumar, Sapna Panwar and Reeta Bhatia
 
Subject Tagetes spp., phenol, liquid culture, rooting, hardening
 
Description Not Available
Marigold is one of the popular ornamental crops grown mostly for loose flower production and garden
display. It is usually propagated through seeds, but some germplasm including male sterile lines (petaloid
and gynomonoecious forms) can only be maintained through vegetative means of propagation. Year round
production and maintenance of true-breeding lines can be possible by employing efficient tissue culture
techniques. However, exudation of phenols from explants and poor ex vitro survival of marigold plants are
the major hindrances. Therefore, the present investigation was carried out with an objective to standardize
the protocol for controlling phenolic exudation from nodal explants and also enhancing the ex vitro survival
of four marigold genotypes viz., Pusa Arpita, Pusa Basanti Gainda, Siracole Orange and Siracole Yellow. The
exudation of phenolic compounds from nodal explants was significantly controlled by incorporating 125 mgl-1
ascorbic acid into the culture induction medium supplemented with BAP (2.0 mgl-1) + NAA (0.1 mgl-1) in all the
genotypes. Marigold micro-shoots cultured on ½ MS liquid rooting medium supplemented with 0.5 mgl-1 IBA
showed highest rooting percentage (99.00%) which was followed by ½ MS + 0.5 mgl-1 NAA (98.75%). Early root induction (5.88 days), longest roots (2.78 cm), moderately high number of roots (47.56) per shoot and highest ex vitro survival (98.75%) were observed with ½ MS + 0.5 mgl-1 NAA. Among the different hardening strategies employed, lowest mortality (11.55%), maximum plant height (15.15 cm) and leaf number (20.95) were noted in plants that were hardened in disposable polypropylene glasses.
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Date 2020-04-15T09:37:11Z
2020-04-15T09:37:11Z
2018-06-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/34803
 
Language English
 
Relation Not Available;
 
Publisher Not Available