Experimental infection of Betanodavirus in freshwater fish Gambusia affinis (Baird and Girard, 1853)—a potential infection model for viral encephalopathy and retinopathy
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Title |
Experimental infection of Betanodavirus in freshwater fish Gambusia affinis (Baird and Girard, 1853)—a potential infection model for viral encephalopathy and retinopathy
Not Available |
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Creator |
J. Praveenraj
P. Ezhil Praveena T. Bhuvaneswari A. Navaneeth Krishnan KP. Jithendran |
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Subject |
Viral nervous necrosis, Betanodavirus,Gambusia affinis,Experimental transmission,RT-PCR.Histopathology, Electron microscopy
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Description |
Not Available
Betanodaviruses are the causative agents of the disease known as viral nervous necrosis (VNN) or viral encephalopathy and retinopathy (VER) in a variety of marine and freshwater fish species. The aim of this study was to demonstrate experimental infection of an isolate of betanodavirus (RGNNV genotype) in freshwater fish,Gambusia affinis,for elucidation of transmission mechanism and potential use as a laboratory model. Morbidity and mortality rate was significantly higher by injection route of infection as compared to immersion by bath and resembled the natural infection of juvenile marine fish.The fish in disease affected group showed severe neurological disorders accompanied by extensive vacuolar degeneration and mild to moderate neuronal necrosis of the brain in comparison to control. Amplification of ~427 bp product in the variable region of the coat protein gene of betanodavirus was achieved by RT-PCR with 100% sequence homology to RGNNV genotype. Not Available |
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Date |
2018-11-15T09:38:58Z
2018-11-15T09:38:58Z 2018-01-02 |
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Type |
Research Paper
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Identifier |
Not Available
0967-6120 http://krishi.icar.gov.in/Publication/handle/123456789/11544 |
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Language |
English
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Relation |
Not Available;
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Publisher |
CIBA
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