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Experimental infection of Betanodavirus in freshwater fish Gambusia affinis (Baird and Girard, 1853)—a potential infection model for viral encephalopathy and retinopathy

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Title Experimental infection of Betanodavirus in freshwater fish Gambusia affinis (Baird and Girard, 1853)—a potential infection model for viral encephalopathy and retinopathy
Not Available
 
Creator J. Praveenraj
P. Ezhil Praveena
T. Bhuvaneswari
A. Navaneeth Krishnan
KP. Jithendran
 
Subject Viral nervous necrosis, Betanodavirus,Gambusia affinis,Experimental transmission,RT-PCR.Histopathology, Electron microscopy
 
Description Not Available
Betanodaviruses are the causative agents of the disease known as viral nervous necrosis (VNN) or viral encephalopathy and retinopathy (VER) in a variety of marine and freshwater fish species. The aim of this study was to demonstrate experimental infection of an isolate of betanodavirus (RGNNV genotype) in freshwater fish,Gambusia affinis,for elucidation of transmission mechanism and potential use as a laboratory model. Morbidity and mortality rate was significantly higher by injection route of infection as compared to immersion by bath and resembled the natural infection of juvenile marine fish.The fish in disease affected group showed severe neurological disorders accompanied by extensive vacuolar degeneration and mild to
moderate neuronal necrosis of the brain in comparison to control. Amplification of ~427 bp product in the variable region of the coat protein gene of betanodavirus was achieved by RT-PCR with 100% sequence homology to RGNNV genotype.
Not Available
 
Date 2018-11-15T09:38:58Z
2018-11-15T09:38:58Z
2018-01-02
 
Type Research Paper
 
Identifier Not Available
0967-6120
http://krishi.icar.gov.in/Publication/handle/123456789/11544
 
Language English
 
Relation Not Available;
 
Publisher CIBA