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Evaluation of Flinders Technology Associates Cards as a Non-lethal Sampling Device for Molecular Diagnosis of Betanodavirus in Asian Seabass, Lates calcarifer (Bloch, 1790)

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Title Evaluation of Flinders Technology Associates Cards as a Non-lethal Sampling Device for Molecular Diagnosis of Betanodavirus in Asian Seabass, Lates calcarifer (Bloch, 1790)
Not Available
 
Creator K Kirti
P. Ezhil Praveena
T. Bhuvaneswari
KP. Jithendran
 
Subject Viral nervous necrosis
Viral encephalopathy and retinopathy
Betanodavirus; Diagnosis
RT-nPCR
FTA elute card
 
Description Not Available
This study aimed to evaluate the utility and efficiency of the Flinders Technology Associates (FTA®) Elute Card
(Whatman®) as a sampling device and storage platform for RNA from Betanodavirus infected biological sample (viz.,cell culture supernatant). The retrieval of target RNA from FTA Elute Card discs with a diameter ranging from 1.2 - 2mm was found to be satisfactory for detection of Betanodavirus by reverse transcription-nested polymerase chain reaction (RT-nPCR). The viral RNA on the cards could be detected by RT-nPCR for a minimum period of 30 days of storage at 4°C, though at lower efficiency after 21 days of storage. In conclusion, FTA cards protocol provides a supplementary method for quick and easy collection of samples, preservation of RNA on a dry storage basis and detection of Betanodavirus infected fish.
Not Available
 
Date 2019-06-12T05:09:48Z
2019-06-12T05:09:48Z
2019-01-21
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/20190
 
Language English
 
Relation Not Available;
 
Publisher Virology: Current Research