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Expression Study of Pluripotency Marker Genes in Gold Fish, Carassius auratrus

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Title Expression Study of Pluripotency Marker Genes in Gold Fish, Carassius auratrus
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Creator Abhay Kumar
Gayatri Tripathi
Bhartendu Vimal
Megha K. Bedekar
A. Pavan Kumar
 
Subject Pluripotency
ICM
Quantitative expression
Goldfish
 
Description Not Available
Aquarium fishes, like medaka, zebrafish and goldfish offer an excellent model to conduct
studies on molecular mechanisms of embryonic developmental pathways, patterns of
genes involved in pluripotency and cell fate decision. To determine the possible candidate
gene as an ideal pluripotency marker in goldfish, the comparative study on quantitative
expression of a set of genes were conducted in the mid-blastula derived inner cell mass
(ICM) and adult somatic cells. Present work explored the expression pattern of four
standard pluripotency genes i.e. Nanog, Oct4, Sox and Pou2 in blastocytes (ICM) as well
as somatic tissues of kidney, liver, heart, brain, muscle and mature gonads of goldfish
using RT-qPCR. Absolute copy number of mRNA transcripts was calculated for each gene
in every tissue. Significantly high (P
Not Available
 
Date 2021-03-11T07:09:08Z
2021-03-11T07:09:08Z
2020-04-10
 
Type Research Paper
 
Identifier Abhay Kumar, Gayatri Tripathi, Bhartendu Vimal, Megha K. Bedekar and Pavan Kumar. A. 2020. Expression Study of Pluripotency Marker Genes in Gold Fish, Carassius auratrus. Int.J.Curr.Microbiol.App.Sci. 9(04): 639-649. doi: https://doi.org/10.20546/ijcmas.2020.904.078
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http://krishi.icar.gov.in/jspui/handle/123456789/46072
 
Language English
 
Relation Not Available;
 
Publisher Excellent Publishers