Record Details

Cloning and Sequence Analysis of 163R and 201R Genes of Camel poxvirus from Indian Dromedaries (Camelus Dromedarius)

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Cloning and Sequence Analysis of 163R and 201R Genes of Camel poxvirus from Indian Dromedaries (Camelus Dromedarius)
Not Available
 
Creator Nagarajan G
Swami SK
Tuteja FC
Sharma G
Mehta SC
Saini N
 
Subject India
Immunomodulatory protein genes
PCR
Cloning
Sequencing
Phylogeny
 
Description Not Available
In the present study, skin scabs were collected from a total number of 10 severely affected Dromedarian camels (Camelus dromedarius) maintained by the Border Security Force (BSF) of Bikaner, Rajasthan an, India. Two immunomodulatory protein genes, 163R and 201R of camelpoxvirus (CMLV) were amplified by Polymerase Chain Reaction using gene specific primers, followed by cloning and sequencing using the standard experimental protocols. CMLV 163R protein is the orthologue of Vaccinia virus A46R protein possessing the VIPER (viral inhibitory peptide of TLR4) sequence (an 11 amino acid peptide-KYSFKLILAEY) at its α1 helix. The RGD motif necessary for mediating the immunomodulatory mechanism is present in the amino acid sequences of CMLV 201R protein. Sequence analysis of both 163R and 201R genes revealed that CMLV obtained from India shared 100% identity with CMLV-Iran and CMLV-Kazakhstan strains both at DNA and protein level. Based on the nucleotide and amino acid residue sequence identities and phylogenetic analyses of these genes, it is found that CMLV-India is forming a cluster with Kazakhstan and Iranian CMLV isolates.
Not Available
 
Date 2020-03-04T04:08:10Z
2020-03-04T04:08:10Z
2017-01-01
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/33523
 
Language English
 
Relation Not Available;
 
Publisher Not Available