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Detection of Mycobacterium avium Subspecies paratuberculosis (MAP) Microorganisms Using Antigenic MAP Cell Envelope Proteins

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Title Detection of Mycobacterium avium Subspecies paratuberculosis (MAP) Microorganisms Using Antigenic MAP Cell Envelope Proteins
Not Available
 
Creator Shanmugasundaram Karuppusamy, Lucy Mutharia, David Kelton, Brandon Plattner, Sanjay Mallikarjunappa, Niel Karrow and Gordon Kirby
 
Subject mycobacterium, cell envelope, ELISA, immunohistochemistry, immunomagnetic separation
 
Description Not Available
Cell envelope proteins from Mycobacterium avium subspecies paratuberculosis (MAP)
that are antigenically distinct from closely related mycobacterial species are potentially
useful for Johne’s Disease (JD) diagnosis. We evaluated the potential of ELISAs, based
on six antigenically distinct recombinant MAP cell envelope proteins (SdhA, FadE25_2,
FadE3_2, Mkl, DesA2, and hypothetical protein MAP1233) as well as an extract of MAP
total cell envelope proteins, to detect antibodies againstMAP in the sera of infected cattle.
The sensitivity (Se) and specificity (Sp) of an ELISA based on MAP total cell envelope
proteins, when analyzing 153 bovine serum samples, was 75 and 96%, respectively.
Analysis of the same samples, using a commercial serum ELISA resulted in a Se of 56%
and Sp of 99%. Results of ELISA analysis using plates coated with recombinant cell
envelope proteins ranged from a highest Se of 94% and a lowest Sp of 79% for Sdh A to
a lowest Se of 67% and a highest Sp of 95% for hypothetical protein MAP1233. Using
polyclonal antibodies to MAP total cell envelope proteins, immunohistochemical analysis
of intestinal and lymph node tissues from JD-positive cattle detected MAP organisms
whereas antibodies to recombinant proteins did not. Finally, polyclonal antibodies to
MAP total cell envelope protein and to recombinant SdhA, FadE25_2, and DesA2
proteins immunomagnetically separated MAP microorganisms spiked in PBS. These
results suggest that antigenically distinct MAP cell envelope proteins and antibodies to
these proteins may have potential to detect MAP infection in dairy cattle.
ICAR
 
Date 2021-07-15T10:25:57Z
2021-07-15T10:25:57Z
2021-02-03
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/48260
 
Language English
 
Relation Not Available;
 
Publisher Frontiers