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Assessment of gamma radiation induced genetic variability in Jatropha curcas using RAPD and DAMD markers

Indian Agricultural Research Journals

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Title Assessment of gamma radiation induced genetic variability in Jatropha curcas using RAPD and DAMD markers
 
Creator BHUMI, SHAH
GAUTAM, SARIPALLI
AKSHAY, ROLA
FENIL, PATEL
FOUGAT, R S
 
Subject DAMD, Genetic diversity, Jatropha curcas, RAPD
 
Description Gamma radiation induced variability among the Jatropha accessions was assessed using morphological and molecularmarkers (RAPD and DAMD) . Various treatments (0, 1, 3, 5, 10, 15, 20, 25, 50 Kr) of gamma radiation doses were givento Jatropha curcas L. genotype Urulikanchan and a total of 32 mutants and 1 control were included in the present studyon the basis of various doses. Some variation in germination% was observed with different doses whereas characters likeheight, girth and canopy diameter showed non significant variation. The 15 RAPD and 4 DAMD primers were used whichproduced polymorphism percentage of 74.23% and 73.68%. PIC values of the markers ranged from 0.732 to 0.904 (RAPD)and 0.765 to 0.868 (DAMD) respectively. RAPD primers OPA 18, OPB 10, OPH 12, OPF 4 and OPI 5 produced bandswhich were specific to some mutants and hence helpful in identifying those mutants. Similarly, DAMD primers, HBV andHRV also produced specific banding profiles. Comparison studies showed very less correlation between the two markersas deduced from the r value (r=0.44). Both the markers proved efficient for polymorphism detection in gamma radiation treated J.curcas mutant.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2013-12-24
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/35965
 
Source The Indian Journal of Agricultural Sciences; Vol 83, No 12 (2013)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/35965/15941
 
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