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Cloning of drought related genes in Vigna aconitifolia through modified differential display

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Title Cloning of drought related genes in Vigna aconitifolia through modified differential display
Not Available
 
Creator Soni P
T. Mohapatra
Bhatt KV
Rizwan M
Sharma R
 
Subject Moth bean
Drought
 
Description Not Available
A total of 12 genotypes of moth bean were evaluated for desiccation tolerance at germination and seedling stage. Differential response was observed at 8% and 15% PEG on germination and seedling level respectively where Varieties RMO-435, RMM-12-Single, RMM12-Poly
And CZM-105 Were foundtolerant. Certain protein were found to be upregulated with treatment in all the samples while some showed upregulation only in tolerant types through SDS PAGE analysis. Twenty four primer combinations of anchored poly T (3) and random primers (8) were used to amplify the transcripts and amplicons were resolved on agarose gel with ethidium bromide. This modified DDRT-PCR assay amplified a total of 95 bands with an average of 4.04 per primer combination. 43 gene fragments were found to be up-regulated whereas 34 were downregulated in response to drought as compared to the control. A total of 23 unigenes (4 contigs and 19 singletons), were derived by cluster assembly and sequence alignment of 29 ESTs Out of 29 ESTs, 4 EST (13.79%) was found to be novel to moth bean. Significant BLASTX similarity (
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Date 2020-12-30T10:44:28Z
2020-12-30T10:44:28Z
2013-05-01
 
Type Research Paper
 
Identifier Soni P, Mohapatra T, Bhatt KV, Rizwan M and Sharma R. 2013. Cloning of drought related genes in Vigna aconitifolia through modified differential display. Journal of cell and Tissue Research. 13(20 3701-3709)
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http://krishi.icar.gov.in/jspui/handle/123456789/44283
 
Language English
 
Relation Not Available;
 
Publisher Not Available