Isolation, purification and characterization of a novel esterase from camel rumen metagenome
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Title |
Isolation, purification and characterization of a novel esterase from camel rumen metagenome
Not Available |
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Creator |
Nilam J.Tulsani
Priyaranjan Mishra Subhash J.Jakhesara Shweta Srivastava Basanti Jyotsana Nishant A.Dafalec Niteen V.Patil Hemant J.Purohit Chaitanya G.Joshia |
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Subject |
Camel rumen
Metagenome Esterase Gene cloning |
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Description |
Not Available
Bacterial esterases are gaining the importance in pharmaceuticals and agrochemical industries due to their excellent biocatalytic properties and a wide range of applications. In the present study, a novel gene encoding an esterase (designated as Est-CR) was identified from shotgun metagenomic sequencing data of camel rumen (Camelus dromedarius) liquor. The open reading frame consisted of 1,224bp, which showed 84.03% sequence identity to Bacteroidales bacterium, corresponding to a protein of 407 amino acids and has a catalytic domain belonging to an esterase. Est-CR belonged to family V with GLSMG domain. The purified enzyme with a molecular mass of 62.64 kDa was checked on SDS-PAGE, and its expression was confirmed by western blotting. The enzyme was active and stable over a broad range of temperature (35–65 °C), displayed the maximum activity at 50 °C and pH 7.0. Individually all metal ions inhibited the enzyme activity, while in combination, K2+, Ca2+, Mg2+ and Mn2+ metal ions enhanced the enzyme activity. The detergents strongly inhibited the activity, while EDTA (10 mM) increased the activity of the Est-CR enzyme. The enzyme showed specificity to short-chain substrates and displayed an optimum activity against butyrate ester. This novel enzyme might serve as a promising candidate to meet some harsh industrial processes enzymatic needs. Not Available |
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Date |
2021-07-23T10:01:26Z
2021-07-23T10:01:26Z 2021-07-14 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/49602 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Elsevier
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