PCR based sensitive detection of Ralstonia solanacearum from soil, eggplant, seeds and weeds.
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Title |
PCR based sensitive detection of Ralstonia solanacearum from soil, eggplant, seeds and weeds.
Not Available |
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Creator |
Ramesh, R., Anthony, J., Jaxon, T.C.D., Gaitonde, S. and Achari, G.
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Subject |
Ralstonia solanacearum
PCR detection soil eggplant seeds weed capsicum |
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Description |
Not Available
Ralstonia solanacearum is an economically important, bacterial plant pathogen which affects a wide range of crop plants. R. solanacearum survives in the soil for many years and weeds serve as symptomless carrier. One of the important aspects in controlling R. solanacearum is its early detection. In this study, detection threshold of R. solanacearum in the soil was standardized using PCR method. The minimum threshold limit ranged between 6.8X10 and 3.6X102 CFU g-1 of soil. Using this standardized protocol R. solanacearum was detected from the rhizosphere soil of eggplants showing varying degrees of wilt. PCR method was quite sensitive to detect R. solanacearum from the xylem fluid of eggplant. Presence of R. solanacearum in the soil infected with capsicum wilt was also demonstrated successfully and the minimum detection limit was 4X102 CFU g-1 of soil. The bacterium was not detected from the eggplant seeds collected during 2006 and 2007 seasons. However, the bacterium was detected from the weed (Alternanthera sessilis) grown in the eggplant field indicating the possibility of weeds serving as symptomless carrier. Using our method, it is possible to detect R. solanacearum from soil, plant and weeds grown in the field at an early stage so that proper management strategies could be taken to prevent the infection and further spread of the pathogen. Not Available |
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Date |
2021-07-22T06:58:03Z
2021-07-22T06:58:03Z 2001-10-13 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/49324 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Taylor and Francis
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