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Expression Profiling of Lipolytic and Long-Chain Fatty Acid Biosynthesis Genes in Catfish Larvae Fed With Graded Lipid Levels

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Title Expression Profiling of Lipolytic and Long-Chain Fatty Acid Biosynthesis Genes in Catfish Larvae Fed With Graded Lipid Levels
Not Available
 
Creator Ishfaq Nazir Mir
P. P. Srivastava
Irfan A Bhat
Jaffer Yousuf Dar
Deepak Agarwal
 
Subject dietary lipid
lipolytic
desaturase
elongase
transcript
Clarias magur
 
Description Not Available
This study was designed to evaluate the effect of dietary lipid levels on the mRNA transcripts of lipid metabolic regulatory genes. Nine combinations of experimental diets containing graded lipid (80, 100, and 120 g/kg) and protein (450, 500, and 550 g/kg) levels were fed to the Clarias magur (Indian walking catfish) larvae from 14 to 35 dph (day post hatching). All the lipolytic genes, such as pancreatic triacylglycerol lipase (PL), lipoprotein lipase (LPL), and bile salt-activated lipase (BAL), and genes for long-chain polyunsaturated fatty acid (LC-PUFA) biosynthetic enzymes like fatty acyl desaturase-2 (FADS2), fatty acyl desaturase-5 (FADS5), and elongase (ELOV) were expressed in a wide range of tissues. A high abundance of mRNA transcript levels of lipolytic genes was detected in the intestine and liver, and similarly, desaturases and elongase were predominantly found to be expressed in the liver, brain, and intestine. Among the diets, a significantly high expression of both lipolytic and LC-PUFA biosynthetic genes were observed at 8% dietary lipid level. The mRNA expression of all the studied genes was down-regulated at 12% dietary lipid contents. Hence, the present study concludes that the efficient nutrient utilization and the lipid metabolic pathway occur at the optimum dietary lipid level of 8% in C. magur larvae.
Not Available
 
Date 2021-07-24T16:50:19Z
2021-07-24T16:50:19Z
2020-09-29
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/49990
 
Language English
 
Relation Not Available;
 
Publisher Frontiers