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Molecular identification of S-alleles associated with self-incompatibility in apple (Malus spp.) genotypes

Indian Agricultural Research Journals

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Title Molecular identification of S-alleles associated with self-incompatibility in apple (Malus spp.) genotypes
 
Creator MIR, JI
AHMED, N
SINGH, D B
SHEEMAR, G
HAMID, ASMA
ZAFFER, SHAFIA
SHAFI, WAJIDA
 
Subject Allele typing, Apple, Incompatibility, S-allele, Pollination management
 
Description Gametophytic self-incompatibility, governed by the S-locus in apple (Malus spp.) plays a vital role for pollination and fruit set. The identification and cloning of RNases has enabled the use of molecular techniques to characterize Sgenotypes in apple cultivars. To identify the S-alleles associated with self-incompatibility, allele specific primers were tested using PCR, evaluating eight apple genotypes. A total of 6 pollen incompatibility groups in apple genotypes were identified among eight accessions by PCR based S-allele typing analysis. Eight putative S-alleles (S1, S2, S7, S19, S21, S23, S24 and S26) were identified with S1S7, S1S23, S1S24, S2S26, S19S24 diploid and S1S21S24 triploid combinations that had not previously been identified from apple cultivars. The molecular allele typing system of Sgenotypes based on PCR is a useful and rapid method for identifying new S-alleles and incompatibility groups in apple and the present results enabled the characterization of eight apple cultivars with respect to S-allele composition whichis an important and preliminary step for pollination management and hybridization in apple breeding programmes.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2016-01-22
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/55221
 
Source The Indian Journal of Agricultural Sciences; Vol 86, No 1 (2016)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/55221/23221
 
Rights Copyright (c) 2016 The Indian Journal of Agricultural Sciences