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Characterization of pigeonpea lipoxygenase in response to Fusarium udum infection*

Indian Agricultural Research Journals

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Title Characterization of pigeonpea lipoxygenase in response to Fusarium udum infection*
 
Creator MANDAL, A K
SINHA, P
 
Subject Fusarium wilt; Lipoxygenase activity; Pigeonpea; Resistance; Steady-state kinetics
 
Description An experiment was conducted during 2005–07 to characterize lipoxygenase activity in pigeonpea in response to Fusarium udum infection. Four known resistant genotypes, viz ‘ICP 87119’, ‘ICP 8863’, ‘BDN 2’ and ‘BSMR 736’ have shown consistently higher lipoxygenase (LOX) activity in comparison to susceptible genotypes viz ‘Bahar’ and ‘TTB 7’. The pH optima for LOX activity were at pH 6–6.5, 7.5 and 9.0 exhibited by 3 distinct peaks. Peaks at pH 6– 6.5 and 7.5 were almost similar in both resistant and susceptible genotype. Peak of higher activity at pH 9.0 was significantly different in resistant in comparison to susceptible genotype. It indicated that pigeonpea LOX system may have at least 3 different isoforms and had substrate preference on linoleic acid. Difference in kinetic parameters like, Km values, catalytic efficiency and selectivity in purified enzyme has supported the differential kinetic behaviour in resistant and susceptible genotype. 
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2011-03-29
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/5288
 
Source The Indian Journal of Agricultural Sciences; Vol 79, No 3 (2009)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/5288/2210
 
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