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Application of PCR based technique for detection of common mastitis pathogens in milk samples of HF crossbred cattle

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Title Application of PCR based technique for detection of common mastitis pathogens in milk samples of HF crossbred cattle
Not Available
 
Creator Jadhav PV
Das DN
Chetana KR
Tarate SB
Shome BR
 
Subject Mastitis
PCR
Staphylococcus epidermidis
Streptococcus agalactiae
Streptococcus dysgalactiae
Escherichia coli
 
Description Not Available
Present study was undertaken to identify the incidence rate for five major pathogens viz., Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Streptococcus agalactiae (S. agalactiae), Streptococcus dysgalactiae (S. dysgalactiae) and Escherichia coli (E. coli) in the milk samples collected from Bangalore and Kolar district by PCR based microorganism detection technique. A total of 214 composite milk samples were screened by CMT and then PCR based detection of pathogens for the samples was carried out. Results revealed presence of S. aureus in 28.5 per cent of the total animals screened, S. epidermis was identified in 15.42 per cent cases. E. coli followed and the incidence rate was 13.55 per cent with regard to E. coli. S. agalactiae and S. dysgalactiae were identified in 10.28 per cent and 3.74 per cent of the cases respectively. None of the pathogens considered in this study were detected in 117 milk samples, these samples were also observed to be normal for CMT. Present study supported the fact that PCR based identification of mastitis causing pathogens from milk is a rapid and reliable method to reveal the exact bacterial etiology of mastitis.
Not Available
 
Date 2018-10-30T11:36:34Z
2018-10-30T11:36:34Z
2013-01-01
 
Type Research Paper
 
Identifier Not Available
2278 – 9103
http://krishi.icar.gov.in/jspui/handle/123456789/8716
 
Language English
 
Relation Not Available;
 
Publisher Society of Science and Nature