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Sequencing and analysis of invariant surface glycoprotein (ISG) gene from Trypanosoma evansi dog isolate

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Title Sequencing and analysis of invariant surface glycoprotein (ISG) gene from Trypanosoma evansi dog isolate
Not Available
 
Creator Rudramurthy GR
Sengupta PP
Lakkundi JN
Ligi M
D'Souza PE
Rahman H
 
Subject Trypanosomosis
Dog isolate
T.evansi
ISG-75
Phylogenetic analysis
 
Description Not Available
Trypanosoma (T) evansi causes a chronic wasting disease called “surra” in cattle, buffaloes, horses, canines, felines etc. In the present study T.evansi isolated from dog from Karnataka state in India was used to sequence invariant surface glycoprotein (ISG) gene. The sequence obtained was analyzed to elucidate its relationship with other isolates/ species. The ISG gene sequences obtained from four recombinant clones revealed the open reading frame (ORF) of 1521 nucleotide (nt) encoding a polypeptide of 506 amino acids (aa) and belongs ISG-75 gene family. Sequence analysis revealed respectively, 92-99% and 65-99% similarity at nucleotide and amino acid levels, with other isolates/ species. Hence, the present studied T.evansi isolate belongs to the RoTat 1.2 strain.
Not Available
 
Date 2018-11-01T08:15:14Z
2018-11-01T08:15:14Z
2015-01-01
 
Type Research Paper
 
Identifier Not Available
2229-791X
http://krishi.icar.gov.in/jspui/handle/123456789/8991
 
Language English
 
Relation Not Available;
 
Publisher Research in Biotechnology