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A pilot sero-survey for surra in livestock in Karnataka by ELISA using flagellar antigen of Trypanosoma evansi

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Title A pilot sero-survey for surra in livestock in Karnataka by ELISA using flagellar antigen of Trypanosoma evansi
Not Available
 
Creator Ligi J
Sengupta PP
Rudramurthy GR
Rahman H
 
Subject Trypanosoma evansi surra
Purified flagellar (FLA) antigen
I- ELISA; sero-epidemiology
 
Description Not Available
Background: Trypanosoma evansi causes trypanosomosis or ‘surra’ in domestic and wild animals and is transmitted by haematophagus tabanid fly. For successful control of the disease, it is important to detect the carrier animals followed by successive treatment. For this purpose a reliable serological test is useful for mass screening of the animals. Methodology: In the present study, emphasis was given to address the need for the development of a mass screening serological test by using purified flagellar antigen of T. evansi for the detection of antibody against surra. Also a pilot sero-epidemiological study for trypanosomosis in different species using purified flagellar (FLA) antigen based indirect enzyme linked immunosorbant assay (I-ELISA) was conducted and compared with standard CATT/T. evansi test. The diagnostic potentiality of purified FLA antigen based I-ELISA (FLA-I-ELISA) was evaluated using 197 sera samples from field animals including cattle, buffaloes, horses and donkeys. Results: The test showed an overall 14.72% sero positivity (S.P). Among cattle, S.P was found to be 18.03%, in buffalo 25%, in horse 6.25% and in donkeys 5.5%. Conclusion: Thus, epidemiological survey were done with purified FLA antigen based I-ELISA test in Karnataka reveals that, buffalo samples showed the maximum sero positivity for trypanosomosis.
Not Available
 
Date 2018-11-03T05:06:06Z
2018-11-03T05:06:06Z
2015-11-01
 
Type Research Paper
 
Identifier Not Available
2278-1404
http://krishi.icar.gov.in/jspui/handle/123456789/9144
 
Language English
 
Relation Not Available;
 
Publisher Bio Med Asia