Record Details

Use of rpoB gene analysis for detection and identification of leptospira species by direct sequencing

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Use of rpoB gene analysis for detection and identification of leptospira species by direct sequencing
Not Available
 
Creator Shivamallu C
Umesha S
Shruthi G
Balamurugan V
 
Subject Histopathological studies
Leptospirosis
Phylogenetic analysis
Polymerase chain reaction
rpoB
 
Description Not Available
Leptospirosis is an emerging disease for which culture and identification are partly unresolved. In fact, 16S rRNA-based sequencing is the most widely used PCR methodology that can detect such uncultivable pathogens. However, this assay has some limitations linked to potential problems of contamination, which hampers diagnosis. To overcome this, we have a simple PCR strategy involving targeting of the gene encoding the RNA polymerase β subunit (rpoB), a highly conserved enzyme. The sequence of the Leptospira rpoB gene was determined and compared with the published sequence. Our findings have significant implications for the development of a new tool for the identification of spirochetes, especially if clinical samples are contaminated or when the infecting strain is uncultivable. The consistent use of PCR has improved the early diagnosis of Leptospirosis but the limitation is that it cannot provide information on the infecting Leptospira strain which provides important epidemiological data.
Not Available
 
Date 2018-11-05T04:09:56Z
2018-11-05T04:09:56Z
2016-02-01
 
Type Research Paper
 
Identifier Not Available
2321-9122
http://krishi.icar.gov.in/jspui/handle/123456789/9284
 
Language English
 
Relation Not Available;
 
Publisher Bioscience Journals