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Assessment of rapid immunochromatographic assay for detection of rabies infection under field condition

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Title Assessment of rapid immunochromatographic assay for detection of rabies infection under field condition
Not Available
 
Creator Chandra V
Shah P
Kapadiya D
Bhanderi B
Dabhi S
Rana S
Manjunathareddy G B
Kanani A
 
Subject Rabies
Immunochromatographic assay
Seller’s stain
FAT
RT-PCR
 
Description Not Available
Rapid and early diagnosis of rabies is of paramount importance in field conditions for control of the
disease as well as for epidemiological surveillance of the prevalence of the disease. Techniques like
Fluorescent Antibody Test and RT-PCR, although highly sensitive and specific, require laboratory
facilities, trained laboratory personnel and are time consuming. Therefore there was an impending need
to evaluate an essay for quick and accurate diagnosis of rabies under field conditions. The present study
was carried out to study the efficacy of a commercially available diagnostic immunochromatographic kit
for detection of rabies antigen from BioNote Inc. Seoul, Korea in comparison with conventional
techniques viz. seller’s stain and FAT as well as sensitive molecular techniques viz. RT-PCR. Also, the
importance of rapid immunodiagnostic test was evaluated for rapid and accurate diagnosis of rabies
under field conditions. A total of 25 brain samples of different species like cattle, buffalo, mongoose and
sloth bear were collected from outbreaks and incidences of rabies in Gujarat State and examined in this
study. Sensitivity of the kit was found to be 100% and specificity was also found to be 100%. Also,
100% agreement was observed between chromatographic assay, seller’s stain, FAT and RT-PCR. The
study implies that the immunochromatographic diagnostic test kit may be employed for diagnosis of
rabies in field conditions.
Not Available
 
Date 2018-11-12T05:13:16Z
2018-11-12T05:13:16Z
2017-04-14
 
Type Research Paper
 
Identifier Not Available
2456-2912
http://krishi.icar.gov.in/jspui/handle/123456789/10269
 
Language English
 
Relation Not Available;
 
Publisher Tropical Medicine and Infectious Disease