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PCR-based Identification of Trypanosoma evansi targeting Internaltranscribed Spacer 1 of rDNA in experimentally infected Mice
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View Archive Info| Field | Value | |
| Title |
PCR-based Identification of Trypanosoma evansi targeting Internaltranscribed Spacer 1 of rDNA in experimentally infected Mice
Not Available |
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| Creator |
Shashi Kant Kankar, Sachin Kumar Goyal, Parvati Sharma, Deepak Kumar Gaur, Souti Prasad Sarkhel and Rajender Kumar
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| Subject |
Trypanosma evansi, Surra, ITS-1, PCR, rDNA, Equine
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| Description |
Not Available
Trypanosoma evansi (T. evansi) is a causative agent of disease called surra, affecting wide range of domestic and wild animals. In this study, a PCR assay was developed using primers targeting ITS-1 region flaking between 5.8 S and 18 S subunits of rRNA. The test was employed using serially diluted DNA extracted from purified trypanosomes ranging from 200 ng/μl to 0.00002 pg/μl and T. evansi infected mice blood ranging from 350 ng/μl to 0.00035 ng/μl. The diagnostic sensitivity of PCR assay was found to be 0.2 pg/μl and 0.035 ng/μl with purified parasite DNA and infected mice blood DNA, respectively. Not Available |
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| Date |
2018-11-22T06:14:22Z
2018-11-22T06:14:22Z 2015-02-04 |
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| Type |
Journal
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| Identifier |
Not Available
0973-9149 http://krishi.icar.gov.in/jspui/handle/123456789/12549 |
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| Language |
English
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| Relation |
Not Available;
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| Publisher |
Indian Journals.com
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