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Antioxidant and antimicrobial activity of ultra-filtered fractions of camel milk protein hydrolysates under in-vitro condition

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Title Antioxidant and antimicrobial activity of ultra-filtered fractions of camel milk protein hydrolysates under in-vitro condition
FUNCTIONAL PROPERTIES OF CAMEL MILK PROTEIN
 
Creator DEVENDRA KUMAR, MANISH KUMAR CHATLI, RAGHVENDAR SINGH, NITIN MEHTA and PAVAN KUMAR
 
Subject Antimicrobial activity
Antioxidant activity
Camel Milk protein
Enzymatic hydrolysis
Ultra filtration
 
Description Not Available
Sequential ultra-filtration technique was used to fractionate camel milk protein hydrolysates products by 3
different proteolytic enzymes, viz. alcalase, α-chymotrypsin and papain. The protein fractions were assessed for
antioxidant activities, viz. 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1
picrylhydrazyl (DPPH) and ferric reducing antioxidant power assay (FRAP), and antimicrobial activity (inhibition
zone assay). The whole hydrolysates recorded significantly higher inhibition activity in ABTS, DPPH and FRAP
assay, whereas among fractions, F2 (1–5 kDa) and F3 (5–10 kDa) of all the 3 hydrolysates had higher activity.
Higher antioxidant activities was also observed in α- chymotrypsin hydrolysates samples and its fractions followed
by alcalase and papain. The zone of inhibition (mm) was also recorded higher for whole hydrolysates as compared
to their fractions; however, different fractions had almost comparable antimicrobial effect. The protein hydrolyastes
with alcalase and α- chymotrypsin recorded comperatively higher antimicrobial activity. The findings suggested
that camel milk proteins could be valuable source to produce protein hydrolysates and ultra-filtration technique
could also be used to get specific molecular weight peptides, however, for application in processed food or for
direct human consumption, use of whole hydrolysates could be more beneficial and cost effective.
Not Available
 
Date 2018-11-29T05:48:38Z
2018-11-29T05:48:38Z
2017-11
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/13981
 
Language English
 
Relation Not Available;
 
Publisher ICAR