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MULTIPLEX-PCR TO DETECT PATHOGENS AND ANALYSIS OF RELATION OF AGE AND STAGE OF LACTATION OF COWS TO SUB-CLINICAL MASTITIS

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Title MULTIPLEX-PCR TO DETECT PATHOGENS AND ANALYSIS OF RELATION OF AGE AND STAGE OF LACTATION OF COWS TO SUB-CLINICAL MASTITIS
Not Available
 
Creator Gaddi RM
Isloor S
Rathnamma D
Avinash B
Veeregowda BM
Bhaskar R
Suguna Rao
 
Subject Sub-clinical mastitis, Somatic cell count, Multiplex-PCR, Predominant bacteria, Age groups, Stage of lactation
 
Description Not Available
In this study, 225 milk samples were collected sequentially during 1st to 88th day from 25 HF cross cows
in an organized farm. First five collections were obtained at a weekly interval (1,7,14,21 and 28 days)
and later, fortnightly for two months (43, 58, 73 and 88 days). These milk samples were screened for
Subclinical mastitis (SCM) by Somatic Cell Count (SCC). Further, multiplex-PCR for detection of
S.aureus, E.coli, S.agalactiae, S.dysgalactiae and S.uberis was employed to detect the major bacterial
pathogens. The SCM positivity was assessed based on criteria of SCC ≥ 500,000 cells /ml. The study
revealed the high prevalence of variable SCM pattern in milking cows by SCC (73.33 %) in sequentially
collected milk samples over a period of 88 days. No specific pattern of prevalence of SCM was
observed during the study period. The prevalence of SCM was not influenced by the stage of lactation.
In all the stages of lactation and age groups S. aureus, Streptococci and E.coli were detected with the
predominance of S. aureus. The varied distribution of organisms in different stages of lactation did not
influence the prevalence of SCM. Further, the high prevalence of SCM was noticed in aged cows.
Among these, maximum number of milk samples (46 %, 52/113) revealing the presence of pathogens
were obtained from cows in the age group 7-11 years. The multiplex PCR was found an easy and rapid
method to detect the predominant pathogens causing SCM. The findings emphasize the need to control
SCM through sequential monitoring of SCM through SCC, multiplex-PCR and proper managemental
practices.
Not Available
 
Date 2019-02-01T06:25:03Z
2019-02-01T06:25:03Z
2016-10-25
 
Type Research Paper
 
Identifier Not Available
2320 – 8694
http://krishi.icar.gov.in/jspui/handle/123456789/16696
 
Language English
 
Relation Not Available;
 
Publisher HORIZON PUBLISHER INDIA [HPI]