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Sequence and structural analysis of OmpW protein of Pasteurella multocida strains reveal evolutionary conservation among members of Pasteurellaceae along with its homologues

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Title Sequence and structural analysis of OmpW protein of Pasteurella multocida strains reveal evolutionary conservation among members of Pasteurellaceae along with its homologues
Not Available
 
Creator Yogisharadhya R
Mohanty NN
Chacko N
Mondal M
Chanda MM
Shivachandra SB.
 
Subject Homogeneity
Pasteurella multocida
Pasteurellaceae
OmpW
Sequence analysis
Structural features
 
Description Not Available
Several bacterial outer membrane proteins (OMPs) are known to play diverse role in physiology including adoptive response to adverse environmental conditions and contribute for enhanced virulence. Pasteurella multocida, a causative agent of multiple infections in animals and avians, is also known to possess several uncharacterized OMPs as virulent factors. In the present study, hitherto uncharacterized OmpW gene encoding for OmpW protein of P. multocidastrains (n = 7) have been cloned, sequenced and comparatively analyzed with existing OmpW sequences/homologues (n = 98) by multiple bioinformatics tools. A comparative phylogenetic, multiple sequence alignment and structural features of OmpW protein of P. multocida with that of homologues in other bacterial species revealed the presence of evolutionarily conserved residues forming a pore like structure in outer membrane. Structurally, OmpW protein of P. multocidahad eight strands (β1-β8) with four external loops (L1–L4). Analysis of transmembrane pore (hydrophobic) features as well as predicted functionality by docking studies using fumerate molecule indicated similarity with that of OmpW protein of E. coli. Further, the study indicated the potential possibilities to target OmpW gene/protein of P. multocida either for development of alternative specific diagnostics or vaccines against pasteurellosis in livestock.
Not Available
 
Date 2019-05-27T06:38:10Z
2019-05-27T06:38:10Z
2018-11-04
 
Type Research Paper
 
Identifier Not Available
2452-0144
http://krishi.icar.gov.in/jspui/handle/123456789/19903
 
Language English
 
Relation Not Available;
 
Publisher Elsevier limited