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Evaluation of a protective antigen gene based SYBR Green I real time PCR for detection of Bacillus anthracis in field samples

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Title Evaluation of a protective antigen gene based SYBR Green I real time PCR for detection of Bacillus anthracis in field samples
Not Available
 
Creator Khandia R.
Pattnaik B.
Rajukumar K.
Pateriya A. K.
Murugkar H. V.
Bhatia S.
Prakash A.
Pradhan H. K.
 
Subject Evaluation of a protective antigen
Detection of Bacillus anthracis
SYBR Green I real time PCR
 
Description Not Available
In the present study, 27 field materials from different sources were tested in SYBR Green I dye based Real Time PCR optimized using WHO recommended conventional PCR primers targeting Bacillus anthracis protective antigen (PA) gene of pX01 plasmid. The assay had detection limit of 0.000002 pg in terms of target DNA quantity and up to 4 copies of pX01 plasmid per reaction, in terms of copy number. Melt curve analysis of PCR products of field samples showed deviation up to 3.2°C in melting temperature from standard anthrax strain suggestive of possible mutation/ mutations in PA gene. Targeted region of PA (596 bp) encompasses domain 2, involved in its binding with lethal factor (LF) and/or edema factor (EF) and mutation in this region may alter the efficacy of PA to traffic toxins inside the cells and results in differed level of virulence of pathogen. The test is useful not only for detection but may be suggestive of mutation also.


Evaluation of a protective antigen gene based SYBR Green I real time PCR for detection of Bacillus anthracis in field samples (PDF Download Available). Available from: https://www.researchgate.net/publication/265013123_Evaluation_of_a_protective_antigen_gene_based_SYBR_Green_I_real_time_PCR_for_detection_of_Bacillus_anthracis_in_field_samples [accessed Jun 3, 2017].
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Date 2018-03-22T07:24:26Z
2018-03-22T07:24:26Z
2013-02-01
 
Type Research Paper
 
Identifier Khandia R, Pattnaik B , Rajukumar K, Pateriya A K, Murugkar H V, Bhatia S, Prakash A and Pradhan H K (2013). Evaluation of a protective antigen gene based SYBR Green I real time PCR for detection of Bacillus anthracis in field samples. Indian Journal of Animal Sciences 83: 118–123.
0367-8318
http://krishi.icar.gov.in/jspui/handle/123456789/5956
 
Language English
 
Relation Not Available;
 
Publisher Department of Biotechnology