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Genetic and antigenic analysis of H5N1 viruses for selection of HA donor virus for vaccine strain

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Title Genetic and antigenic analysis of H5N1 viruses for selection of HA donor virus for vaccine strain
Not Available
 
Creator Bhatia, S.
Kunal, A.
Khandia, R.
Siddiqui, A.
Pateriya, A. K.
Sood, R.
 
Subject Avian influenza
Antigenic cartography
H5N1
Vaccine
 
Description Not Available
Genetic and antigenic analysis of H5N1 viruses, isolated in India during a period from year 2006 to 2010, was carried out for selection of the potential H5-HA (haemagglutinin) gene donor virus for developing a reverse genetics based DIVA marker H5 vaccine for poultry in India. Out of the 47 H5N1 viruses (clade 2.2), 14 representative viruses were selected on the basis of amino acid sequence analysis of HA1 gene for further antigenic characterization. Using antigenic cartography, an antigenic map was constructed based on the data of cross-HI (haemagglutinin inhibition) titration of 14 sera versus 14 viruses to visualize the relatedness among the antigens and antigenic coverage of the sera. Sera against five H5N1 viruses (A/crow/Assam/142119/2008, A/chicken/West Bengal/100879/2008, A/chicken/West Bengal/155505/2009, A/chicken/West Bengal/80995/2008 and A/chicken/West Bengal/81760/2008) exhibited maximum (100 %) antigenic coverage, hence, were selected as the potential HA donor viruses. However, the virus strain A/chicken/West Bengal/80995/2008 matched completely with the consensus amino acid sequence of the 47 viruses, therefore, was considered the best HA donor candidate out of the five showing 100 % antigenic coverage. The present study demonstrates a stepwise methodology for logical selection of vaccine strain or HA gene donor strain for developing H5 vaccines using genetic and antigenic data.
Not Available
 
Date 2018-03-22T07:25:13Z
2018-03-22T07:25:13Z
2013-12-01
 
Type Research Paper
 
Identifier Bhatia, S., Kunal, A., Khandia, R., Siddiqui, A., Pateriya, A. K. and Sood, R. (2013). Genetic and antigenic analysis of H5N1 viruses for selection of HA donor virus for vaccine strain. Indian Journal of Virology.Indian J Virol. 2013 Dec; 24(3): 357–364.
0970-2822
http://krishi.icar.gov.in/jspui/handle/123456789/5962
 
Language English
 
Relation Not Available;
 
Publisher Indian Virological Society