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Detection of bovine viral diarrhoea virus (BVDV) in peripheral blood mononuclear cells (PBMCs) of BVDV antibody positive Indian cattle following mitogen stimulation

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Title Detection of bovine viral diarrhoea virus (BVDV) in peripheral blood mononuclear cells (PBMCs) of BVDV antibody positive Indian cattle following mitogen stimulation
Not Available
 
Creator Gupta, V.
Mishra, N.
Rajukumar, K.
Behera, S. P.
Dubey, S.P.
 
Subject Bovine viral diarrhoea
BVDV
PBMCs
Indian cattle
 
Description Not Available
The aim of this study was to determine the in vitro permissivity of peripheral blood mononuclear cells (PBMCs) from bovine viral diarrhea virus (BVDV)-immune field cattle to homologous and heterologous BVDVs. PBMCs from seventeen BVDV-naïve and sixteen BVDV-immune animals were infected with noncytopathic BVDV-1 or BVDV-2. The immune status of cattle was indicated by the presence of virus neutralizing antibodies, while viral load of PBMCs was determined by real-time RT-PCR. The results revealed that the PBMCs from naïve or immune animals were permissive to either BVDV-1 or BVDV-2, but the viral load was significantly higher for the naïve than for the immune animals. Furthermore, the load of homologous virus in PBMCs from immune animals was lower than that of heterologous virus. Our results provide evidence that the PBMCs from BVDV-immune cattle in field are susceptible to reinfection with homologous or heterologous BVDV, albeit to a lower extent in the former case.
PMID: 24957715
Not Available
 
Date 2018-03-22T07:48:17Z
2018-03-22T07:48:17Z
2014-02-01
 
Type Research Paper
 
Identifier Gupta, V., Mishra, N., Rajukumar, K., Behera, S. P., Dubey, P. (2014). Detection of bovine viral diarrhoea virus (BVDV) in peripheral blood mononuclear cells (PBMCs) of BVDV antibody positive Indian cattle following mitogen stimulation. Ind J Anim Sci. 84: 156-158.
0367-8318
http://krishi.icar.gov.in/jspui/handle/123456789/5970
 
Language English
 
Relation Not Available;
 
Publisher Indian Council of Agricultural Research.