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Development and evaluation of a truncated recombinant NS3 antigen-based indirect ELISA for detection of pestivirus antibodies in sheep and goats

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Title Development and evaluation of a truncated recombinant NS3 antigen-based indirect ELISA for detection of pestivirus antibodies in sheep and goats
Not Available
 
Creator Kalaiyarasu S.
Mishra N.
Rajukumar K.
Nema R.K.
Behera S.P.
 
Subject BVDV antibodies
NS3 ELISA
VNT
Goat
Sheep
 
Description Not Available
The aim of this study was to develop an indirect ELISA using the helicase domain of bovine viral diarrhoea virus (BVDV) NS3 protein instead of full-length NS3 protein for detection of BVDV and BDV antibodies in sheep and goats and its validation by comparing its sensitivity and specificity with virus neutralization test (VNT) as the reference test. The purified 50 kDa recombinant NS3 protein was used as the coating antigen in the ELISA. The optimal concentration of antigen was 320 ng/well at a serum dilution of 1:20 and the optimal positive cut-off optical density value was 0.40 based on test results of 418 VNT negative sheep and goat sera samples. When 569 serum samples from sheep (463) and goats (106) were tested, the ELISA showed a sensitivity of 91.71% and specificity of 94.59% with BVDV VNT. A good correlation (93.67%) was observed between the two tests. It showed a sensitivity of 85% and specificity of 86.6% with VNT in detecting BDV antibody positive or negative samples. This study demonstrates the efficacy of truncated recombinant NS3 antigen based ELISA for seroepidemiological study of pestivirus infection in sheep and goats.
Not Available
 
Date 2018-03-22T07:50:52Z
2018-03-22T07:50:52Z
2014-08-01
 
Type Research Paper
 
Identifier Kalaiyarasu S, Mishra N, Rajukumar K, Nema RK, Behera SP. (2015). Development and evaluation of a truncated recombinant NS3 antigen-based indirect ELISA for detection of pestivirus antibodies in sheep and goats. J Immunoassay Immunochem. 2015;36(3):312-23
1532-1819
http://krishi.icar.gov.in/jspui/handle/123456789/5991
 
Language English
 
Relation Not Available;
 
Publisher Taylor & Francis