Record Details

siRNAs targeting PB2 and NP genes potentially inhibit replication of Highly Pathogenic H5N1 Avian Influenza Virus

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title siRNAs targeting PB2 and NP genes potentially inhibit replication of Highly Pathogenic H5N1 Avian Influenza Virus
Not Available
 
Creator Behera P.
Nagarajan S.
Murugkar H.V.
Kalaiyarasu S.
Prakash A.
Gothalwal R.
Dubey S.C.
Kulkarni D.D.
Tosh C.
 
Subject Avian influenza virus
NP
PB2
siRNA
subtype H5N1
 
Description Not Available
Highly Pathogenic Avian Influenza (HPAI) H5N1 virus is a threat to animal and public health worldwide. Till date, the H5N1 virus has claimed 402 human lives, with a mortality rate of 58% and has caused the death or culling of millions of poultry since 2003. In this study, we have designed three siRNAs (PB2-2235, PB2-479 and NP-865) targeting PB2 and NP genes of avian influenza virus and evaluated their potential, measured by hemagglutination (HA), plaque reduction and Real time RT-PCR assay, in inhibiting H5N1 virus (A/chicken/Navapur/7972/2006) replication in MDCK cells. The siRNAs caused 8- to 16-fold reduction in virus HA titers at 24 h after challenged with 100TCID50 of virus. Among these siRNAs, PB2-2235 offered the highest inhibition of virus replication with 16-fold reduction in virus HA titer, 80% reduction in viral plaque counts and 94% inhibition in expression of specific RNA at 24 h. The other two siRNAs had 68–73% and 87–88% reduction in viral plaque counts and RNA copy number, respectively. The effect of siRNA on H5N1 virus replication continued till 48h (maximum observation period). These findings suggest that PB2-2235 could efficiently inhibit HPAI H5N1 virus replication.
Not Available
 
Date 2018-03-22T07:51:19Z
2018-03-22T07:51:19Z
2015-06-01
 
Type Research Paper
 
Identifier Behera P, Nagarajan S, Murugkar HV, Kalaiyarasu S, Prakash A, Gothalwal R, Dubey SC, Kulkarni DD, Tosh C (2015) siRNAs targeting PB2 and NP genes potentially inhibit replication of Highly Pathogenic H5N1 Avian Influenza Virus.J Biosci. 2015 Jun;40(2):233-40.
0250-5991
http://krishi.icar.gov.in/jspui/handle/123456789/5993
 
Language English
 
Relation Not Available;
 
Publisher Indian Academy of Sciences