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Genetic and antigenic characterization of H5N1 viruses of clade 2.3.2.1 isolated in India

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Title Genetic and antigenic characterization of H5N1 viruses of clade 2.3.2.1 isolated in India
Not Available
 
Creator Bhat S.
Bhatia S.
Pillai A.S.
Sood R.
Singh V.K.
Shrivas O.P.
Mishra S.K.
Mawale N.
 
Subject Antigenic cartography
Growth kinetics
HPAI H5N1
Phylogenetic analysis
 
Description Not Available
The recurrent circulation of highly pathogenic avian influenza (HPAI) H5N1 in Indian poultry since 2006 resulted in emergence of the viruses of distinct antigenic clades of haemagglutinin (HA) with the majority of the H5N1 outbreaks since 2011 belonging to clade 2.3.2.1. The present study was aimed to characterize the antigenic profile of a collection of H5N1 HPAI viruses of clade 2.3.2.1 isolated in India by applying antigenic cartography, serological data and phylogenetic analysis. Eleven H5N1 viruses (2 of clade 2.2 and 9 of clade 2.3.2.1) were selected based on genetic analysis and were further characterized by antigenic cartography analysis based on cross HI (hemagglutination inhibition) data. This study highlights the intercladal antigenic differences between clades 2.3.2.1 and 2.2 and the intracladal antigenic divergence among the clade 2.3.2.1 viruses. Five viruses of clade 2.3.2.1 were also studied for analysis of glycosylation pattern of Hemagglutinin (HA) gene and the growth kinetics analysis in MDCK cells in which the viruses CL03485/H5N1 and 03CL488/H5N1 showed better replication kinetics than other viruses. The study presents a baseline data of antigenicity and other factors that can be used in the selection of suitable H5 vaccine strains or HA donor viruses to develop H5 vaccine strains by reverse genetics or other methods for control of currently circulating H5N1 viruses in Indian region.
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Date 2018-03-22T07:51:54Z
2018-03-22T07:51:54Z
2015-11-01
 
Type Research Paper
 
Identifier Bhat S, Bhatia S, Pillai AS, Sood R, Singh VK, Shrivas OP, Mishra SK, Mawale N. (2015). Genetic and antigenic characterization of H5N1 viruses of clade 2.3.2.1 isolated in India. Microb Pathog. 2015 Nov;88:87-93.
0882-4010
http://krishi.icar.gov.in/jspui/handle/123456789/5997
 
Language English
 
Relation Not Available;
 
Publisher Elsevier B.V.