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Host miRNA bi-specifically regulates RIG-I and influenza virus replication

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Title Host miRNA bi-specifically regulates RIG-I and influenza virus replication
Not Available
 
Creator Ingle H.
Kumar S.
Raut A.A.
Mishra A.
Kulkarni D.D.
Kameyana T.
Takaoka A.
Akira S.
Kumar H.
 
Subject MicroRNAs
Retinoic acid
RIG-1
miR-485
 
Description Not Available
MicroRNAs (miRNAs) are small noncoding RNAs that are responsible for dynamic changes in gene expression, and some regulate innate antiviral responses. Retinoic acid–inducible gene I (RIG-I) is a cytosolic sensor of viral RNA; RIG-I activation induces an antiviral immune response. We found that miR-485 of the host was produced in response to viral infection and targeted RIG-I mRNA for degradation, which led to suppression of the antiviral response and enhanced viral replication. Thus, inhibition of the expression of mir-485 markedly reduced the replication of Newcastle disease virus (NDV) and the H5N1 strain of influenza virus in mammalian cells. Unexpectedly, miR-485 also bound to the H5N1 gene PB1 (which encodes an RNA polymerase required for viral replication) in a sequence-specific manner, thereby inhibiting replication of the H5N1 virus. Furthermore, miR-485 exhibited bispecificity, targeting RIG-I in cells with a low abundance of H5N1 virus and targeting PB1 in cells with increased amounts of the H5N1 virus. These findings highlight the dual role of miR-485 in preventing spurious activation of antiviral signaling and restricting influenza virus infection.
Not Available
 
Date 2018-03-22T07:52:36Z
2018-03-22T07:52:36Z
2015-12-01
 
Type Research Paper
 
Identifier Ingle H, Kumar S, Raut AA, Mishra A, Kulkarni DD, Kameyana T, Takaoka A, Akira S and Kumar H. (2015). Host miRNA bi-specifically regulates RIG-I and influenza virus replication. Sci Signal. 2015 Dec 8;8(406):ra126.
1525-8882
http://krishi.icar.gov.in/jspui/handle/123456789/6003
 
Language English
 
Relation Not Available;
 
Publisher American Association for the Advancement of Science