Elevated level of pro inflammatory cytokine and chemokine expression in chicken bone marrow and monocyte derived dendritic cells following LPS induced maturation
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Title |
Elevated level of pro inflammatory cytokine and chemokine expression in chicken bone marrow and monocyte derived dendritic cells following LPS induced maturation
Not Available |
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Creator |
Kalaiyarasu S.
Bhatia S. Mishra N. Sood R. Kumar M. SenthilKumar D. Bhat S. Dass Prakash M. |
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Subject |
CXCLi2
Cytokine Dendritic cell Lipopolysaccharide Surface marker Toll like receptors iNOS |
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Description |
Not Available
The study was designed to characterize and compare chicken bone marrow and peripheral blood monocyte derived dendritic cells (chBM-DC and chMoDC) and to evaluate inflammatory cytokine and chemokine alterations in response upon LPS stimulation. Typical morphology was observed in DCs from 48h of culture using recombinant chicken GM-CSF and IL-4. Maturation of DCs with LPS (1μg/ml) showed significant up regulation of mRNA of surface markers (CD40, CD80, CD83, CD86, MHC-II and DC-LAMP (CD208)), pro-inflammatory cytokines (IL-1β, IL-6, TNF-α (LITAF)), iNOS, chemokine CXCli2 and TLRs4 and 15. Basal level of TLR1 mRNA expression was higher followed by TLR15 in both DCs irrespective of their origin. Expression of iNOS and CXCLi2 mRNA in mature DCs of both origins were higher than other surface molecules and cytokines studied. Hence, its level of expression can also be used as an additional maturation marker for LPS induced chicken dendritic cell maturation along with CD83 and CD40. LPS matured DCs of both origins upregulated IL-12 and IFN-γ. Based on CD40 and CD83 mRNA expression, it was observed that LPS induced the maturation in both DCs, but chMoDCs responded better in expression of surface markers and inflammatory mediator genes. Not Available |
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Date |
2018-03-22T07:53:56Z
2018-03-22T07:53:56Z 2016-09-01 |
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Type |
Research Paper
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Identifier |
Kalaiyarasu S, Bhatia S, Mishra N, Sood R, Kumar M, SenthilKumar D, Bhat S, Dass Prakash M.(2016). Elevated level of pro inflammatory cytokine and chemokine expression in chicken bone marrow and monocyte derived dendritic cells following LPS induced maturation.Cytokine. 2016 Sep;85:140-7.
1043-4666 http://krishi.icar.gov.in/jspui/handle/123456789/6013 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Elsevier B.V.
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