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RT LAMP assay: an alternative approach for profiling of bovine heat shock protein 70 gene in PBMC cultured model

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Title RT LAMP assay: an alternative approach for profiling of bovine heat shock protein 70 gene in PBMC cultured model
Not Available
 
Creator Sengar G S, Deb R*, Raja T V, Singh U, Kant R , Bhanuprakash V., Alyethodi R R, Kumar S, Verma P , Chakraborty S, Alex R, Singh R
 
Subject Absorbance
Hsp70
RT-LAMP
RT-PCR
 
Description Not Available
The purpose of this study is to develop a novel
Reverse Transcriptase Loop-mediated isothermal amplification
(RT-LAMP) based assay for in vitro profiling of heat
shock protein 70 (Hsp70) in bovine peripheral blood mononuclear
cell (PBMC) culture model utilizing the absorbance
level of magnesium pyrophosphate—a by-product of
LAMP reaction. A set of bovine Hsp70 specific RT-LAMP
primers were designed to detect the differential absorbance
level of magnesium pyrophosphate by-product which signifies
the degree of Hsp70 amplification from cDNA of
thermally induced cultured cells at different recovery periods.
The study revealed significant (P < 0.05) correlation
between absorbance level and the fold change of Hsp70
transcripts at different kinetic intervals of heat stress recovery
in bovine PBMC cell culture models. RT-LAMP based
absorbance assay can be used as an indicator to measure
the degree of bovine Hsp70 transcripts produced during
thermal stress and can be used as an alternative to the traditional
Real time PCR assay. Developed RT-LAMP assay
can be used as a cost-effective method for profiling of
bovine HSP70 gene.
Science and Engineering Research Board, Government of India (YSS/2014/000279)
 
Date 2017-07-01T04:55:14Z
2017-07-01T04:55:14Z
2017-05-27
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/4561
 
Language English
 
Relation Not Available;
 
Publisher Springer