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Microbiological efficacy of pressure assisted thermal processing and natural extracts against Bacillus amyloliquefaciens spores suspended in deionized water and beef broth

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Title Microbiological efficacy of pressure assisted thermal processing and natural extracts against Bacillus amyloliquefaciens spores suspended in deionized water and beef broth
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Creator Suresh Devatkal
Jeremy Somerville
Rarinthorn Thammakulkrajang
V.M. Balasubramaniam
 
Subject Pressure-assisted-thermal processing
Natural extracts
 
Description Not Available
Effect of aqueous extracts of pomegranate peel (PPE) and tamarind pulp (TPE) on lethality of pressure assisted thermal processing (PATP) against Bacillus amyloliquefaciens Fad 82 spores was investigated. B.amyloliquefaciens spores (≈108 CFUml−1) were suspended in deionized
water (DIW), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer PPE, and TPE (1% w/v in DIW, pH-6) and beef broth with or without PPE and TPE (0.45% w/v). Samples were subjected to pressure assisted thermal processing (PATP, 600 MPa, 105 ◦C), and thermal processing (TP; 0.1 MPa, 105 ◦C) for 0 (come-up time), 0.5, 1, 3, and 5min. Beef broth samples
were also pressure treated (HPP; 600 MPa, 35 ◦C) for 3 and 5min. Dormant spore survivors were enumerated by spread-plate technique. Heat shock (80 ◦C for 15min) was used to enumerate the heat sensitive spores. A 5-min PATP reduced spore survivors counts to below 1-logCFUml−1 (≈6.5–7.0 log reduction) in PPE and TPE as compared to 2.5-log and 3.1-log in
DIW and HEPES buffer, respectively. 3min PATP treatment reduced the spore survival to 3.58 and 2.1 logCFUml−1 in beef broth and beef broth+natural extract, respectively. TP and HPP processing could inactivate the spores only by 0.5–1.2 logCFUml−1. PPE and TPE are found
to enhance the efficacy of PATP.
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Date 2017-07-20T08:49:51Z
2017-07-20T08:49:51Z
2015-01-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/4837
 
Language English
 
Relation Not Available;
 
Publisher Elvevier