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Characterization of TLR expression in Staphylococcus aureus induced mastitis in mice model by probe based real time PCR

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Title Characterization of TLR expression in Staphylococcus aureus induced mastitis in mice model by probe based real time PCR
Not Available
 
Creator Mitra SD
Ghosh SK
Krishnamoorthy P
Chakraborty A
Nimita CV
Roy M
Shome BR
Rahman H
 
Subject Bovine mastitis, Intramammary infection, Staphylococcus aureus, Toll like receptors
 
Description Not Available
Staphylococcus aureus infection of the bovine mammary gland can result in a spectrum of clinical outcomes
ranging from acute to chronic and subclinical. A molecular understanding of the principles causing this is particularly important to eventually develop innovative strategies for prevention and treatment in mastitis. The present study characterized the temporal (2, 4, 8, 12, 24 and 48h) expression of 5 important toll like receptors (TLR 2, TLR 4,
TLR 9, TLR 11 and TLR 12) induced by S. aureus intramammary inoculation (IMI) in established mice model
employing probe based real time PCR (RT-PCR). S. aureus challenge provoked transcriptional expression of each of the 5 TLRs in a temporal manner with maximum expression at 8 h after IMI. S. aureus maximally altered the mRNA concentration of TLR 9 and TLR 12 (> 5 fold) over the PBS control. We found TLR2 to be weakly (1- to 2-fold) regulated by the S. aureus challenge in this study. This study emphasized the contribution of other TLRs besides the most commonly studied TLR 2 and TLR 4 in activating immune response by switching on several receptors mediated signalling cascades. This study sheds light into the diverse pathogen recognition receptor (PRR) mediated mechanisms involved in host pathogen interaction.
Not Available
 
Date 2018-09-15T05:22:04Z
2018-09-15T05:22:04Z
2014-10-31
 
Type Research Paper
 
Identifier Not Available
0367-8318
http://krishi.icar.gov.in/jspui/handle/123456789/6870
 
Language English
 
Relation Not Available;
 
Publisher Indian Council of Agricultural Research