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Amide Side Chain Amphiphilic Polymers Disrupt Surface Established Bacterial Bio-films and Protect Mice from Chronic Acinetobacter baumannii Infection

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Title Amide Side Chain Amphiphilic Polymers Disrupt Surface Established Bacterial Bio-films and Protect Mice from Chronic Acinetobacter baumannii Infection
Not Available
 
Creator Uppu DSSM
Samaddar S
Ghosh C
Krishnamoorthy P
Shome BR
Haldar J
 
Subject Anti-infective biomaterials Biofilm disruption Membrane-active amphiphilic polymer In-vivo burn wound infection Acinetobacter baumannii Bacterial resistance
 
Description Not Available
Bacterial biofilms represent the root-cause of chronic or persistent infections in humans. Gram-negative
bacterial infections due to nosocomial and opportunistic pathogens such as Acinetobacter baumannii are
more difficult to treat because of their inherent and rapidly acquiring resistance to antibiotics. Due to
biofilm formation, A. baumannii has been noted for its apparent ability to survive on artificial surfaces for
an extended period of time, therefore allowing it to persist in the hospital environment. Here we report,
maleic anhydride based novel cationic polymers appended with amide side chains that disrupt surface
established multi-drug resistant A. baumannii biofilms. More importantly, these polymers significantly
(p < 0.0001) decrease the bacterial burden in mice with chronic A. baumannii burn wound infection. The
polymers also show potent antibacterial efficacy against methicillin resistant Staphylococcus aureus
(MRSA), vancomycin resistant Enterococci (VRE) and multi-drug resistant clinical isolates of A. baumannii
with minimal toxicity to mammalian cells. We observe that optimal hydrophobicity dependent on the
side chain chemical structure of these polymers dictate the selective toxicity to bacteria. Polymers
interact with the bacterial cell membranes by causing membrane depolarization, permeabilization and
energy depletion. Bacteria develop rapid resistance to erythromycin and colistin whereas no detectable
development of resistance occurs against these polymers even after several passages. These results
suggest the potential use of these polymeric biomaterials in disinfecting biomedical device surfaces after
the infection has become established and also for the topical treatment of chronic bacterial infections.
Not Available
 
Date 2018-09-15T07:07:42Z
2018-09-15T07:07:42Z
2015-09-30
 
Type Research Paper
 
Identifier 0142-9612
http://krishi.icar.gov.in/jspui/handle/123456789/6888
 
Language English
 
Relation Not Available;
 
Publisher Elsevier Publications