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Evaluation of antiviral activity of Ocimum sanctum and Acacia arabica leaves extracts against H9N2 virus using embryonated chicken egg model.

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Title Evaluation of antiviral activity of Ocimum sanctum and Acacia arabica leaves extracts against H9N2 virus using embryonated chicken egg model.
Not Available
 
Creator Ghoke, S. S.
Sood, R.
Kumar, N.
Pateriya, A. K.
Bhatia, S.
Mishra, A.
Dixit, R.
Singh, V. K.
Desai, D. N.
Kulkarn, D. D
Dimri, U.
Singh, V. P.
 
Subject A. arabica; Avian influenza H9N2; HA; O. sanctum; Real time RT-qPCR; in ovo testing.
 
Description Not Available
In the view of endemic avian influenza H9N2 infection in poultry, its zoonotic potential and emergence of antiviral resistance, two herbal plants, Ocimum sanctum and Acacia arabica, which are easily available throughout various geographical locations in India were taken up to study their antiviral activity against H9N2 virus. We evaluated antiviral efficacy of three different extracts each from leaves of O. sanctum (crude extract, terpenoid and polyphenol) and A. arabica (crude extract, flavonoid and polyphenol) against H9N2 virus using in ovo model.

METHODS:
The antiviral efficacy of different leaves extracts was systematically studied in three experimental protocols viz. virucidal (dose-dependent), therapeutic (time-dependent) and prophylactic (dose-dependent) activity employing in ovo model. The maximum non-toxic concentration of each herbal extracts of O. sanctum and A. arabica in the specific pathogen free embryonated chicken eggs was estimated and their antiviral efficacy was determined in terms of reduction in viral titres, measured by Haemagglutination (HA) and real time quantitative reverse transcription polymerase chain reaction (RT-qPCR) assays.

RESULTS:
All the extracts of O. sanctum (crude extract, terpenoid and polyphenol) and A. arabica (crude extract, flavonoid and polyphenol) showed significant virucidal activity, however, crude extract ocimum and terpenoid ocimum showed highly significant to significant (p < 0.001-0.01) decrease in virus genome copy numbers with lowest dose tested. Similarly, therapeutic effect was observed in all three extracts of O. sanctum in comparison to the virus control, nevertheless, crude extract ocimum and terpenoid ocimum maintained this effect for longer period of time (up to 72 h post-incubation). None of the leaves extracts of A. arabica had therapeutic effect at 24 and 48 h post-incubation, however, only the crude extract acacia and polyphenol acacia showed delayed therapeutic effect (72 h post-inoculation). Prophylactic potential was observed in polyphenol acacia with highly significant antiviral activity compared to virus control (p 
Not Available
 
Date 2018-09-24T05:35:49Z
2018-09-24T05:35:49Z
2018-06-05
 
Type Journal
 
Identifier Ghoke, S. S ,Sood, R. ,Kumar, N. ,Pateriya, A. K. ,Bhatia, S. ,Mishra, A. ,Dixit, R. ,Singh, V. K. ,Desai, D. N. ,Kulkarn, D. D. ,Dimri, U. ,Singh, V. P..Evaluation of antiviral activity of Ocimum sanctum and Acacia arabica leaves extracts against H9N2 virus using embryonated chicken egg model. BMC Complementary and Alternative Medicine.18(1):174-184.
29866088
http://krishi.icar.gov.in/jspui/handle/123456789/7195
 
Language English
 
Relation Not Available;
 
Publisher Blackwell Verlag GmbH