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Meat Species Identification by Randomly Amplified Polymorphic DNA - Polymerase Chain Reaction

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Title Meat Species Identification by Randomly Amplified Polymorphic DNA - Polymerase Chain Reaction
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Creator Girsh P.S, A.R Sen, S. Vaithiyanathan, Y. Babji, R. Rajith & M. Muthukumar
 
Subject Meat, adulteration, DNA, PCR, RAPD.
 
Description Not Available
Misrepresentation of meat for economic gains is a common economic fraudulence prevalent across the
world. In the present study, a molecular technique based on Randomly Amplified Polymorphic DNA
Polymerase Chain Reaction (RAPD - PCR) was tried for differentiation of meat of cattle (Bos indicus),
buffalo (Bubalus bubalis), sheep (Ovis aries), goat (Capra hircus) and chicken (Gallus gallus). RAPD
profile of cattle revealed four amplicons of size 148, 234, 363 & 452 bp, whereas, that of buffalo revealed
amplicons of size 148, 273, 345 & 429 bp. Whereas, sheep and goat yielded three (147, 645 & 389 bp) and
four amplicons (145, 375, 613 & 777 bp), respectively. Chicken RAPD profile indicated four amplicons of
size 263, 351, 467 & 689 bp. Results indicated that, RAPD PCR can be of use in differentiation of meat
from different meat animals which can be employed for authentication of meat and prevention of
misrepresentation of meat.
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Date 2018-11-17T04:40:56Z
2018-11-17T04:40:56Z
2011-12-31
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/11649
 
Language English
 
Relation Not Available;
 
Publisher Journal of Meat Science