Effect of concentration and addition method of glycerol on the quality of cryopreserved mithun (Bos frontalis) spermatozoa
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Title |
Effect of concentration and addition method of glycerol on the quality of cryopreserved mithun (Bos frontalis) spermatozoa
sperm cryopreservation glycerol mithun |
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Creator |
Baruah KK
Dhali A Mech A Bora B Das J Bora R Mondal M Sarmah BC Deka BC Rajkhowa C |
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Subject |
sperm cryopreservation glycerol mithun
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Description |
Not Available
The effect of concentration and addition method of glycerol on the quality of cryopreserved mithun (Bos frontalis) spermatozoa was investigated. Semen samples were collected from five healthy mithun bulls through rectal massage method and cryopreserved in liquid nitrogen. The samples were diluted in Tris–egg yolk–glycerol extender, equilibrated for 4 h at 4 °C and loaded into 0.50‐ml straws. The straws were then frozen in liquid nitrogen vapour for 10 min and finally plunged into liquid nitrogen for storage. The required amount of glycerol was added into the diluted samples either in a single dose (3%, 4%, 5%, 6% or 7%; added at 37 °C immediately before equilibration) or in split doses (5%, 6% or 7%; the total amount was divided into four equal parts, and a part was added at 37 °C immediately before equilibration, and the remaining parts were added subsequently at 1, 2 and 3 h of equilibration at 4 °C). In the single‐dose addition method, following freeze‐thawing, greater (p Not Available |
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Date |
2018-11-15T04:54:13Z
2018-11-15T04:54:13Z 2013-12-01 |
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Type |
Research Paper
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Identifier |
Baruah KK, Dhali A, Mech A, Bora B, Das J, Bora R, Mondal M, Sarmah BC, Deka BC and Rajkhowa C. 2013. Effect of concentration and addition method of glycerol on the quality of cryopreserved mithun (Bos frontalis) spermatozoa. Journal of Animal Physiology and Animal Nutrition, 97:1051-1058.
Not Available http://krishi.icar.gov.in/Publication/handle/123456789/11357 |
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Language |
English
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Relation |
97;6
;1051-58rrr |
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Publisher |
Not Available
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