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Effect of concentration and addition method of glycerol on the quality of cryopreserved mithun (Bos frontalis) spermatozoa

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Title Effect of concentration and addition method of glycerol on the quality of cryopreserved mithun (Bos frontalis) spermatozoa
sperm cryopreservation glycerol mithun
 
Creator Baruah KK
Dhali A
Mech A
Bora B
Das J
Bora R
Mondal M
Sarmah BC
Deka BC
Rajkhowa C
 
Subject sperm cryopreservation glycerol mithun
 
Description Not Available
The effect of concentration and addition method of glycerol on the quality of cryopreserved mithun (Bos frontalis) spermatozoa was investigated. Semen samples were collected from five healthy mithun bulls through rectal massage method and cryopreserved in liquid nitrogen. The samples were diluted in Tris–egg yolk–glycerol extender, equilibrated for 4 h at 4 °C and loaded into 0.50‐ml straws. The straws were then frozen in liquid nitrogen vapour for 10 min and finally plunged into liquid nitrogen for storage. The required amount of glycerol was added into the diluted samples either in a single dose (3%, 4%, 5%, 6% or 7%; added at 37 °C immediately before equilibration) or in split doses (5%, 6% or 7%; the total amount was divided into four equal parts, and a part was added at 37 °C immediately before equilibration, and the remaining parts were added subsequently at 1, 2 and 3 h of equilibration at 4 °C). In the single‐dose addition method, following freeze‐thawing, greater (p 
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Date 2018-11-15T04:54:13Z
2018-11-15T04:54:13Z
2013-12-01
 
Type Research Paper
 
Identifier Baruah KK, Dhali A, Mech A, Bora B, Das J, Bora R, Mondal M, Sarmah BC, Deka BC and Rajkhowa C. 2013. Effect of concentration and addition method of glycerol on the quality of cryopreserved mithun (Bos frontalis) spermatozoa. Journal of Animal Physiology and Animal Nutrition, 97:1051-1058.
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http://krishi.icar.gov.in/Publication/handle/123456789/11357
 
Language English
 
Relation 97;6
;1051-58rrr
 
Publisher Not Available