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Dietary supplementation of n-3 polyunsaturated fatty acid alters endometrial expression of genes involved in prostaglandin biosynthetic pathway in breeding sows (Sus scrofa)

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Title Dietary supplementation of n-3 polyunsaturated fatty acid alters endometrial expression of genes involved in prostaglandin biosynthetic pathway in breeding sows (Sus scrofa)
 
Creator Gokuldas PP
Singh SK
Tamuli MK
Naskar S
Vashi Y
Thomas R
Barman K
Pegu SR
Chethan SG
Agarwal SK
 
Subject Dietary supplementation
Endometrial expression
Prostaglandin
n-3 PUFA
sow
 
Description Not Available
The present investigation was designed to study the effect of dietary supplementation of omega-3 (n-3)
PUFA on endometrial expression of fertility-related genes in breeding sows. Sixteen crossbred sows were
randomized to receive diets containing 4% (wt/wt) flaxseed oil as n-3 PUFA source (TRT group) or isonitrogenous, iso-caloric standard control diet (CON group), starting from the first day of estrus up to 40 days and were artificially bred on the second estrus. Endometrial samples were collected during days 10e11 and 15e16 post-mating for studying relative expression profile of candidate genes viz. Prostaglandin F Synthase (PGFS), microsomal Prostaglandin E Synthase-1 (mPGES-1) and Carbonyl Reductase-1 (CBR-1) using quantitative Real-Time PCR. Expression level of mPGES-1 gene transcript was 2.1-fold higher (P < 0.05) during 10e11 days of pregnancy and 1.4-fold higher (P > 0.05) during 15e16 days of pregnancy in TRT group as compared to CON group. Relative expression of PGFS gene transcript was significantly lower (P < 0.05) during 10e11 days of pregnancy in TRT group while there was no significant effect (P > 0.05) of dietary supplementation during 15e16 days of pregnancy. Endometrial mRNA level of CBR1 was significantly lower (P < 0.05) with 3.93-fold decrease in TRT group during 10e11 days of pregnancy whereas 2.82-fold reduction in expression (P > 0.05) was observed subsequently during 15 e16 days of pregnancy as compared to CON group. Collectively, these results indicate that dietary n-3 PUFA supplementation can modulate gene expression of key enzymes in prostaglandin biosynthetic pathway during early gestation, which in turn might have beneficial impact on overall reproductive response in breeding sows. These findings partly support strategic dietary supplementation of plant-based source of n-3 PUFA with an aim to improve overall reproductive performance in sows.
Not Available
 
Date 2018-12-06T05:15:38Z
2018-12-06T05:15:38Z
2018-04-01
 
Type Research Paper
 
Identifier 1
0093-691X
http://krishi.icar.gov.in/jspui/handle/123456789/15591
 
Language English
 
Relation Not Available;
 
Publisher Elsevier